Chemistry: molecular biology and microbiology – Vector – per se
Patent
1995-01-09
1998-06-09
Elliott, George C.
Chemistry: molecular biology and microbiology
Vector, per se
435 9131, 435375, 514 44, 935 32, C12N 1585, C12N 1586, C12N 1563, C12N 701
Patent
active
057632685
ABSTRACT:
Hepatitis delta is used as a vector for inhibition of viral infection and to express proteins in vivo in a cell-specific manner. The scope of delta's use as a vector is broadened in the present invention in several important ways. For example, a delta RNA genome capable of self-replication is enlarged to carry additional information, either coding for messenger RNA for a protein, or for a targeted ribozyme, which can be delivered to liver cells using delta's normally infectious properties, or to other cell types using chimeric delta viral agents carrying altered surface proteins. In another embodiment, the delta vector is made self-limiting, so that its role in delivering targeted information is separated from its viral property of unlimited infectious replication. Targeting is achieved through the use of sequences flanking the delta sequences that have affinity for sites on RNA to be cleaved.
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George Shaji T.
Goldberg Allan R.
Robertson Hugh D.
Elliott George C.
Innovir Laboratories, Inc.
Larson Thomas G.
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