Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Reexamination Certificate
2006-10-27
2010-02-02
Nickol, Gary B (Department: 1648)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Reexamination Certificate
active
07655394
ABSTRACT:
The invention provides a novel assay for NS2/3 cleavage detection, particularly, a novel assay for the detection of the NS3 cleavage product in the presence of uncleaved NS2/3 based on discrimination of the activity of the resulting NS3 protease product from the NS3 protease activity of the NS2/3 uncleaved protein. Certain reaction conditions allow this discrimination in situ without having to resort to physical separation of both proteases by decreasing the NS3 protease activity of the NS2/3 protein and increasing the NS3 protease activity of the resulting NS3 protease product, thereby producing a “signal window” upon which it is feasible to distinguish and measure NS3 protease activity produced from the cleavage of NS2/3.
REFERENCES:
patent: 5739002 (1998-04-01), DeFrancesco et al.
patent: 6251583 (2001-06-01), Zhang et al.
patent: 6815159 (2004-11-01), Thibeault et al.
patent: 2002/0177725 (2002-11-01), Priestley
patent: 2002/0192640 (2002-12-01), Thibeault et al.
patent: 11124400 (1999-07-01), None
patent: WO 97/08304 (1997-03-01), None
patent: WO 01/68818 (2001-09-01), None
patent: WO 02/16379 (2002-02-01), None
patent: WO 02/48375 (2002-06-01), None
Welbourn et al., Hepatitis C Virus NS2/3 Processing is Required for NS3 Stability and Viral RNA Replication, Journal of Biological Chemistry Papers in Press, Jun. 24, 2005, Manuscript M505019200.
“IUPAC-IUB Commission on Biochemical Nomenclature Symbols for Amino-Acid Derivatives and Peptides Recommendations (1971)”; Biochemistry, vol. 11, No. 9, p. 1726, 1972.
A. A. Kolykhalov, et al. “Hepatitis C Virus-Encoded Enzymatic Activities and Conserved RNA Elements in the 3′ Nontranslated Region are Essential for Virus Replication in Vivo”, J. of Virology, vol. 74, No. 4, p. 2046-2051, 2000.
D. Thibeault, et al. “In Vitro Characterization of a Purified NS2/3 Protease Variant of Hepatitis C Virus”, J. of Biological Chemistry, vol. 276, No. 49, p. 46678-46684, 2001.
M. Pallaoro, et al. “Characterization of the Hepatitis C Virus NS2/3 Processing Reaction by Using a Purified Precursor Protein”, J. of Virology, vol. 75, No. 20, p. 9939-9946, 2001.
E. Bianchi, et al., “Synthetic Depsipeptide Substrates for the Assay of Human Hepatitis C Virus Protease”, Analytical Biochemistry, vol. 237, p. 239-244, 1996.
S. R. LaPlante, et al., “Solution Structure of Subtrate-based Ligands when Bound to Hepatitis C Virus NS3 Protease Domain”, J. of Biological Chemistry, vol. 274, No. 26, p. 18618-18624, 1999.
P.L. Darke, et al., “Inhibition of Hepatitis C Virus NS2/3 Processing by NS4A Peptides”, J. Biological Chemistry, vol. 274, No. 49, p. 34511-34514, 1999.
C. Lin, et al., “A Central Region in the Hepatitis C Virus NS4A Protein Allows Formation of an Active NS3-NS4A Serine Proteinase Complex In Vivo and In Vitro”, J. of Virology, vol. 69, No. 7, p. 4373-4380, 1995.
A. Urbani, et al., “Multiple Determinants Influence Complex Formation of the Hepatitis C Virus NS3 Protease Domain with Its NS4A Cofactor Peptide”, Biochemistry, vol. 38, p. 5206-5215, 1999.
D. S. Waugh, “Making the Most of Affinity Tags”, Trends in Biotechnology, vol. 23, No. 6, p. 316-320, 2005.
C. Steinkuhler, et al., “Activity of Purified Hepatitis C Virus Protease NS3 on Peptide Substrates”, J. of Virology, vol. 70, No. 10, p. 6694-6700, 1996.
J-H. Zhang, et al., “A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays”, J. of Biomolecular Screening, vol. 4, No. 2, p. 67, 1999.
J.C. Ferreon, et al., “Molecular Determinants of TRIF Proteolysis Mediated by the Hepatitis C Virus NS3/4A Protease”, J. Biological Chemistry, vol. 280, No. 21, pp. 20483-20492, 2005.
J.O. Koch, et al., “In Vitro Studies on the Activation of the Hepatitis C Virus NS3 Proteinase by the NS4A Cofactor”, Virology, vol. 221, p. 54-66, 1996.
L. Pieroni, et al., “In Vitro Study of the NS2-3 Protease of Hepatitis C Virus”, J. of Virology, vol. 71, No. 9, p. 6373-6380, 1997.
M. M. Martin, et al., “Single-Cell Resolution Imaging of Membrane-Anchored Hepatitis C Virus NS3/4A Protease Activity”, Biological Chemistry, vol. 387, p. 1075, 2006.
A. Berger, et al., “Mapping the Active Site of Papain with the Aid of Peptide Substrates and Inhibitors”, Phil. Trans. Roy. Soc. London, B257, p. 249-264, 1970.
Maurice Roger
Thibeault Diane
Boehringer Ingelheim International GmbH
Devlin Mary-Ellen M.
Dow David A.
Kinsey White Nicole
Morris Michael P.
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