Hepatitis C virus asialoglycoproteins

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Virus or component thereof

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4241851, 435 5, 435 692, 435 699, 530395, 530826, F61K 3929, C12P 2162, C12Q 170, C07K 100

Patent

active

059422343

ABSTRACT:
Two Hepatitis C Virus envelope proteins (E1 and E2) are expressed without sialylation. Recombinant expression of these proteins in lower eukaryotes, or in mammalian cells in which terminal glycosylation is blocked, results in recombinant proteins which are more similar to native HCV glycoproteins. When isolated by GNA lectin affinity, the E1 and E2 proteins aggregate into virus-like particles.

REFERENCES:
patent: 5350671 (1994-09-01), Houghton et al.
Lanford et al., "Analysis of Hepatitis C Virus Capsid, E1, and E2/NS1 Proteins Expressed in Insect Cells," Virology 197:225-235 (1993).
Spaete et al., "Characterization of the Hepatitis C Virus E2/NS1 Gene Product Expressed in Mammalian Cells," Virology 188:819-830 (1992).
Koff, "A redoubtable obstacle to a Hepatitis C vaccine," Gastroenterology 104:1228-1229 (1993).
Farci et al., "Lack of protective immunity against reinfection with Hepatitis C virus," Science 258:135-140 (1992).
Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology (W.B. Saunders Company, Philadelphia) p. 138 (1987).
Goochee et al., "The oligosaccharides of glycoproteins: bioprocess factors affecting oligosaccharide structure and their effect on glycoprotein properties," Bio/Technology 9:1347-1353 (1991).

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