Hepatitis B vaccine

Details Hepatitis B vaccine Hepatitis B vaccine

1984-05-29

1985-11-19

Rose, Shep K.

Drug, bio-affecting and body treating compositions

Lymphokine

210635, 210656, 435239, A61K 3929

Patent

active

045541574

ABSTRACT:
Immunogenically active fragments of hepatitis B surface antigen, which are a glycoprotein of molecular weight about 28,000 (gp 28) and a protein of molecular weight about 23,000 (p 23), obtained by known methods by treatment of surface antigen with detergent, are prepared in micelle form substantially free from detergent. The micelles are at least as immunogenic as the unfragmented antigen from which they are derived and are useful in the preparation of hepatitis B vaccine. The detergent is removed by layering the detergent containing fragmentation product on an aqueous buffer containing a sucrose gradient and centrifuging the layered buffer when micelles containing both gp 28 and p 23 form and can be recovered in substantially detergent free form from the centrifuged layered buffer.

REFERENCES:
patent: 4186193 (1980-01-01), McAleer et al.
Skelly et al., J. Gen. Virol. 44 679-689, (1979).
Helenius et al., Biochem. Biophys. Acta 436, 895-899, (1976).
Morein et al., Nature, 276, 715-718, (1978).
Simons et al., P.N.A.S. 75, No. 11, 5306-5310, (1978).
Zuckerman, New Scientist, Oct. 16th, 1980, 167-168.
Gerin et al., J. Virol. 7, No. 5, 569-576, (1971).
Sukeno et al., J. Virol. 9, No. 1, 182-183, (1972).

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