Heparinase gene from flavobacterium heparinum

Details Heparinase gene from flavobacterium heparinum Heparinase gene from flavobacterium heparinum

1991-10-23

1998-02-03

Prouty, Rebecca E.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 2, 435 691, 435 698, 435 712, 4351723, 435232, 4353201, 935 10, 935 14, 935 27, 935 56, 935 72, C12N 1560, C12N 121, C12N 988

Patent

active

057143765

ABSTRACT:
The cloning of the heparinase gene from Flavobacterium Heparinum using the polymerase chain reaction is described. The Open Reading Frame (ORF) corresponded to 1152 base pairs encoding a precursor protein of MW 43,800 daltons. The amino acid sequence reveals a 20-residue leader peptide. The gene was expressed in two expression systems in E. coli.

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