Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1993-11-15
1994-08-16
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4352521, 435850, C12N 924, C12N 112, C12N 100
Patent
active
053386779
ABSTRACT:
A heparinase derived from Flavobacterium heparinum which meets all requirements for a clinical reagent that can eliminate heparin interference of normal blood function has been developed. The heparinase, derived from Flavobacterium heparinum, is free of a component that inhibits coagulation wherein the anticoagulant component does not bind, and the heparinase does bind, to a polysulfated resin having a pH of 7.0 and a conductivity between 3 and 12 mmhos. It is stable under normal manufacturing, shipping and clinical storage conditions for at least one year. The heparinase in useful in vitro to eliminate the interference in hematological assays due to the presence of heparin. The heparinase is also useful for the in vivo neutralization of heparin during surgical procedures. Advantages of this enzyme are that it achieves neutralization faster and more completely than previously available enzymes and is stable for long periods of time.
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Heft Robert A.
Lewis N. Tracey
Zimmermann Joseph J.
Ibex Technologies, Inc.
Meller Michael V.
Naff David M.
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