Hemoglobins with intersubunit dislufide bonds

Details Hemoglobins with intersubunit dislufide bonds Hemoglobins with intersubunit dislufide bonds

1995-05-26

2000-09-26

Carlson, Karen Cochrane

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4353201, 435325, 4352523, 536 231, 530385, C12P 2106, C07H 1700, C07K 14805

Patent

active

061241148

ABSTRACT:
Cysteine substitution mutants of alpha and/or beta globin mutants are produced by recombinant DNA techniques and used in the construction, intracellularly or otherwise, of mutant hemoglobins in which alpha- and beta-globin like subunits are crosslinked by disulfide bonds. Solutions of these mutant hemoglobins are used as blood substitutes.
Preferably, these mutant hemoglobins contain further mutations which reduce their affinity for oxygen.
Hemoglobins are preferably obtained by recombinant DNA techniques. Both alpha and beta globin chains can now be readily expressed, making possible the commercial production of wholly artificial hemoglobin, whether conventional or mutant in form. Solutions of wholly artificial hemoglobins are also used as blood substitutes. Expression of the alpha glolbin gene was substantially improved by means of a beta globin gene "header".

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