Hemoglobin purification

Details Hemoglobin purification Hemoglobin purification

1993-07-02

1995-04-18

Kim, Sun Uk

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210639, 210645, 210782, 210789, 436177, 436178, 530385, 530412, 530422, 530427, B01D 1100, B01D 6100, B01D 2126, A61K 3514

Patent

active

054075793

ABSTRACT:
Disclosed is an improved method for hemoglobin purification utilizing a novel two-step dual-aqueous-phase extraction technique to separate hemoglobin from red cell membrane stroma and other protein contaminants. In the first step, a first dual-aqueous-phase liquid system is prepared which comprises an upper aqueous phase containing polyethylene glycol in water and a lower aqueous phase containing a phosphate buffer at a pH of about 10. In the second step, a second dual-aqueous-phase liquid system is prepared which comprises an upper aqueous phase, which contains the polyethylene glycol phase containing the hemoglobin extracted in the first step, and a lower aqueous phase containing a new phosphate buffer at a pH of less than 7.5. After the second extraction step, purified hemoglobin solution can then be obtained by removing the phosphate salt and the minute amounts of polyethylene glycol contained in the lower phase.

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