Hemoglobin preparation with increased oxygen release

Details Hemoglobin preparation with increased oxygen release Hemoglobin preparation with increased oxygen release

1977-04-21

1979-01-23

Phillips, Delbert R.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

260112B, C07C10352

Patent

active

041360931

ABSTRACT:
Hemoglobin preparation suited for intravenous injection with increased oxygen release in relation to erythrocytes or hemoglobin solution, comprising a substantially pyrogen-free condensation product of hemoglobin and pyridoxal phosphate having a retention time in the blood vessel from about 2 to 9 hours. It is produced by washing human erythrocytes at least four times with a weakly alkaline solution, then hemolyzing and treating the material with a cation exchange resin in the H+ form until the pH value has dropped to about 5 to 5.5, separating the material from resin and any precipitated stroma, diluting the material to a hemoglobin concentration of about 5 to 9%, adjusting the pH to about 7 to 9, displacing any oxygen therein, and adding about 0.25 to 1.25 g of pyridoxal-5-phosphate per liter of hemoglobin solution of 5 to 9% concentration. To increase the retention time still further there can be included the further steps of treating the solution with a borohydride and then with a dialdehyde thereby to cross-link the hemoglobin molecules in said erythrocytes, and separating uncross-linked hemoglobin from the residual solution of the desired cross-linked hemoglobin.

REFERENCES:
patent: 4001200 (1977-01-01), Bonsen et al.
Benesch, et al., Biochemistry 11, 1972, pp. 3576-3582.
Benesch, et al., Biochem. and Biophys. Res. Commun. 63, 1975, pp. 1123-1129.

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