Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal
Patent
1981-11-16
1984-02-14
Schain, Howard E.
Chemistry of carbon compounds
Miscellaneous organic carbon compounds
C-metal
2601125R, 424101, 426647, 426657, A23J 106, C07G 700, C07C10352
Patent
active
044315813
DESCRIPTION:
BRIEF SUMMARY
Slaughtering blood contains baluable components, namely protein having a high nutritive value and functional properties, which make it useful as texturing agent in foodstuffs, and further heme which is a sought-after iron-enriching agent.
The hemoglobin, which contains the main part of the protein in the blood, has a taste of iron because of the heme. It is therefore of interest to develop a method for cleaving the hemoglobin into an iron-free globin and a heme concentrate, and in such a way that the functional properties of the protein and the heme are preserved so as to give these products a high value.
As is well-known, protein is easily denatured and therewith looses its functional properties, for example, when treated at a high pH (over 9 to 10). The denaturation can be counteracted by operating at a low temperature, less than 0.degree. C.
In order that heme may be useful as a pharmaceutical, i.e. in order that the iron be present in a readily absorbable form, the heme must be present in native form. In hemoglobin, the heme is present in native form out of which the iron, as krown, is readily absorbed.
Out of conventional iron preparations, such as iron sulfate, only a fraction of the iron is absorbed because certain substances present in the food (e.g., phytates) prevent the absorption. This drawback is not present in iron based on heme.
Heme has a pronounced tendency to polymerize and to form difficultly soluble aggregates out of which the iron cannot be absorbed. Known methods for cleaving the hemoglobin into heme and globin and for separating the heme by extraction with acetone or methyl ethyl ketone result in a polymerized heme.
The present invention realtes to a method for recovering a heme concentrate from a mixture of heme and blood substance obtained when cleaving hemoglobin.
Heme, iron heme, is composed of iron and protoporphyrin (see, for example, Textbook of Biochemistry, West & Todd, MacMillan Co., 1961, p. 533) and constitutes the prosthetic group in hemoglobin and amounts to 3.8 percent by weight based on the hemoglobin.
As starting material, a heme product is preferably used which is obtained by cleaving hemoglobin into heme and globin.
The cleavage can be carried out in different ways, for example, by treating hemoglobin in an acid or alkaline milieu.
The raw material can comprise, for example, whole blood, red blood cells, or hemoglobin in another milieu.
A particularly suitable starting material is the heme product obtained according to the method described in the Swedish Patent No. 7407882-5 and in the Swedish Patent Application No. 7513987-3. According to this method, the hemoglobin is cleaved in an aqueous ethanol solution having an ethanol content of at least 40 percent by volume, at a pH of less than 4.5. The precipitate of heme product formed thereby is separated. The remaining globin solution is practically free of heme.
This known product contains about 10 percent by weight of heme while the rest consists of other blood substance, most likely mainly of protein. Moreover, this heme product, in all probability, contains blood substances, such as lipoproteins, lipides, phosphatidyl compounds and cholesterol.
According to the new method, a heme concentrate is obtained having a heme content of about 40 percent by weight. The composition is not known. This heme concentrate is intended for use as a pharmaceutical against anemia and and as an iron-enriching agent in foodstuffs.
The blood protein, mainly globin, which is simultaneously recovered, is intended for purposes of food-stuffs and fodder.
The method is technically and economically feasibly on industrial scale.
According to the method according to the invention, said heme raw material is treated with a dehydrating agent, preferably a lower alcohol or a mixture of alcohols and/or ketones and esters, said treatment taking place either
(a) at a pH of at least 8.0, preferably 9.0 to 10.5, or
(b) in the presence of a compound of the formula: ##STR1## where the substituents can have the following meanings: --R (R is a saturated or
REFERENCES:
patent: 4098780 (1978-07-01), Lindroos
patent: 4330463 (1982-05-01), Luijerink
International J. of Biochemistry, vol. 4, No. 21 (1973), pp. 259-267, Wakid et al.
Journal of Histochemistry and Cytochemistry, vol. 22, No. 9 (1974), pp. 908-910, Straus.
The Proteins, vol. II, Part A, pp. 328-329.
Acta Med. Scand. Suppl. 629, 1980, Reizenstein, pp. 7-47.
Organic Chemistry, 1951, Holliman, p. 529.
Handbook of Chemistry & Physics, p. 170.
Porphyrins & Metalloporphyrns, pp. 829-833, Smith Nutrition Reports International, May, 1976, vol. 13, p. 330.
Textbook of Biochemistry, West et al., pp. 493 & 500.
Practical Physiological Chem., Hawk et al., 1954, p. 469.
LandOfFree
Heme concentrate and method for the preparation thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Heme concentrate and method for the preparation thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Heme concentrate and method for the preparation thereof will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2374503