Hematopoietic stem cells and methods of treatment of...

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus

Reexamination Certificate

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C435S455000, C435S320100

Reexamination Certificate

active

07153501

ABSTRACT:
Isolated, mammalian, bone marrow-derived, lineage negative hematopoietic stem cell populations (Lin−HSC) contain endothelial progenitor cells (EPC) capable of forming retinal blood vessels. At least about 50% of the cells in the isolated Lin−HSC population include cell surface markers for CD31 and c-kit. Up to about 8% of the cells can include the Sca-1 cell marker, and up to about 4% of the cells can include the Flk-1/KDR marker. The isolated Lin−HSC populations of the present invention are useful for treatment of ocular vascular diseases. The isolated Lin−HSC populations that have been transfected with therapeutically useful genes are also provided, which are useful for delivering genes to the eye for cell-based gene therapy.

REFERENCES:
patent: 6767727 (2004-07-01), Glucksmann et al.
patent: 2003/0017564 (2003-01-01), Schimmel et al.
Smith, L.E.H. Stem cells go for the eyes. Nature Medicine 8:932, 2002.
Otani et al. Bone marrow-derived stem cells target retinal astrocytes and can promote or inhibit retinal angiogenesis. Nature Medicine 8:1004-1010, 2002.
McFarland et al. Gene therapy for proliferative ocular diseases. Expert Opin. Biol. Ther. 4: 1053-1058, 2004.

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