Hematopoietic stem cell specific gene expression

Details Hematopoietic stem cell specific gene expression Hematopoietic stem cell specific gene expression

1992-12-15

1996-09-17

Zitomer, Stephanie W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

4352402, 4353201, C07H 2104, C12N 510, C12N 1585

Patent

active

055569544

ABSTRACT:
The subject application relates to a hematopoietic stem cell specific promoter and to a hematopoietic stem cell specific enhancer and to uses therefor. The invention also includes a hematopoietic stem cell specific promoter-heterologous gene construct, where the expression of the heterologous gene is under the control of the myeloid cell type specific promoter. Additionally, the application is drawn to a method of expressing a heterologous gene in a hematopoietic stem cell. The invention further includes a method of expressing a selected heterologous gene product in hematopoietic stem cells in an individual. The invention also includes a transgenic non-human mammal in which the hematopoietic stem cells express a protein encoded by a heterologous gene. Finally the invention includes a method for identifying factors which can regulate hematopoietic stem cell specific transcription.

REFERENCES:
patent: 4959313 (1990-10-01), Taketo
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Nolta et al., Exp. Hematol. 20(9): 1065-1071 (1992).
Day, The Washington Post, "Washington Business" Section, Nov. 7, 1994.
Pahl, Heike L. et al., "Optimization of Transient Transfection into Human Myeloid Cell Lines Using a Luciferase Reporter Gene," Exp. Hematol. 19:1038-1041 (1991).
Arnaout, M. Amin et al., "Amino Acid Sequence of the Alpha Subunit of Human Leukocyte Adhesion Receptor Mol (Complement Receptor Type 3)," J. of Cell Biology 106:2153-2158 (1988).
Shelley, C. Simon et al., "The promoter of the CD11b gene directs myeloid-specific and developmentally regulated expression," Proc. Natl. Acad. Sci. USA 88:10525-10529 (1991).
Pahl, H. L. et al., "Characterization of the Myeloid-Specific CD11b Promoter," Blood 79(4):865-870 (1992).
Pahl, H. L. et al., "The CD11B Promoter Directs Monocytic expression of a Reporter Gene in Transgenic Mice," The American Society of Hematology, 33rd Annual Meeting, ASH Abstract (1991).
Satyabhama, S. et al., "Short-Term Efficient Expression of Transfected DNA in Human Hematopoietic Cells by Electroporation: Definition of Parameters and Use of Chemical Stimulators," DNA, 7(3):203-209 (1988).
Brown, J. et al., "The gene encoding the stem cell antigen, CD34, is conserved in mouse and expressed in haemopoietic progenitor cell lines, brain, and embryonic fibroblasts," Int. Immunol., 3:175-184 (1991).

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