Helper virus-free AAV production

Details Helper virus-free AAV production Helper virus-free AAV production

2005-08-22

2008-10-21

Guzo, David (Department: 1636)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Introduction of a polynucleotide molecule into or...

C435S320100, C435S455000

Reexamination Certificate

active

07439065

ABSTRACT:
A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.

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