Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2006-11-07
2006-11-07
Housel, James (Department: 1648)
Chemistry: molecular biology and microbiology
Vector, per se
C435S235100, C435S455000, C435S456000, C435S457000, C435S173300, C536S023100
Reexamination Certificate
active
07132277
ABSTRACT:
The present invention features helper-dependent adenoviral vector elements, and helper adenoviral elements, that enhance the production and isolation of helper-dependent adenoviral vectors. Such elements include a modified packaging signal having low homology to, and preferably less activity than, a wild-type packaging signal, an E4 non-coding segment directly joined to the 5′ ITR that confers a selective advantage, and stuffer region(s) that provide a helper-dependent adenoviral vector with a GC content of about 50% to about 60%. The modified packaging signal is preferably used in a helper virus to decrease recombination and generation of the virus. The E4 non-coding segment and the stuffer region(s) are preferably used in a helper-dependent adenoviral vector to provide the vector with a growth advantage over a helper virus.
REFERENCES:
patent: 5464758 (1995-11-01), Gossen et al.
patent: 5650298 (1997-07-01), Bujard et al.
patent: 5654182 (1997-08-01), Wahl et al.
patent: 5874534 (1999-02-01), Vegeto et al.
patent: 5919676 (1999-07-01), Graham et al.
patent: WO 93/23431 (1993-11-01), None
patent: WO 96/40955 (1996-12-01), None
patent: WO 97/00326 (1997-01-01), None
patent: WO 97/32481 (1997-09-01), None
patent: WO97/32481 (1997-09-01), None
patent: WO 97/48806 (1997-12-01), None
patent: WO 98/13510 (1998-04-01), None
Grable et al., 1992 Journal of Virology vol. 66 (2), pp. 723-731.
Hierholzer et al., “Adenoviruses from Patients with AIDS: A Plethora of Serotypes and a Description of Five New Serotypes of Subgenus D (Types 43-47)”, The Journal of Infectious Diseases, vol. 158, No. 4, pp. 804-813 (Oct. 1998).
Hitt et al., “Human Adenovirus Vectors for Gene Transfer into Mammalian Cells”, Advances in Pharmacology, vol. 40, pp. 137-206 (1997).
Mitani et al., “Rescue, propagation, and partial purification of a helper virus-dependent adenovirus vector”, Proc. Natl. Acad. Sci. USA, vol. 92, pp. 3854-3858 (Apr. 1995).
Fisher et al., “Recombinant Adenovirus Deleted of AllViral Genes for Gene Therapy of Cystic Fibrosis”, Virology, vol. 217, pp. 11-22 (1996).
Kochanek et al., “A new adenoviral vector: Replacement of all viral coding sequences with 28 kb of DNA independently repressing both full-length dystrophin and β-galactosidase”, Proc. Natl. Acad. Sci USA, vol. 93, pp. 5731-5736 (Jun. 1996).
Parks et al., “A helper-dependent adenovirus vector system: Removal of helper virus by Cre-mediated excision of the viralpackaging signal”, Applied Biological Sciences, vol. 93, pp. 13565-13570 (1996).
Parks et al., “A Helper-Dependent System for Adenovirus Vector Production Helps Define a Lower Limit for Efficient DNA Packaging”, Journal of Virology, vol. 71, No. 4, pp. 3293-3298 (Apr. 1997).
Schiedner et al., “Genomic DNA transfer with a high-capacity adenovirus vector results in improvedin vivogene expression and decreased toxicity”, Nature Genetics, vol. 18, pp. 180-183 (Feb. 1998).
Arnold et al., “The promoter of the chicken cardiac myosin light chain 2 gene shows cell-specific expression in transfected primary cultures of chicken muscle”, Nucleic Acids Research, vol. 16, No. 6, pp. 2411-2429 (1988).
Johnson et al., “Muscle Creatine Kinase Sequence Elements Regulating Skeletal and Cardiac Muscle Expression in Transgenic Mice”, Molecular and Cellular Biology, vol. 9, No. 8, pp. 3393-3399 (Aug. 1989).
Wang et al., “Positive and negative regulation of gene expression in eukaryotic cells with an inducible ftranscriptional regulator”, Gene Therapy, vol. 4, pp. 432-441 (1997).
Schmid et al., “Bipartite Structure and Functional Independence of Adenovirus Type 5 Packaging Elements”, Journal of Virology, vol. 71, No. 5, pp. 3375-3384 (May 1997).
Morsy et al., “An adenoviral vector deleted for all viral coding sequences results in enhanced safety and extended expression of a leptin transgene”, Proc. Natl. Acad. Sci. USA, vol. 95, pp. 7866-7871 (Jul. 1998).
Chartier et al., “Efficient Generation of Recombinant Adenovirus Vectors by Homologous Recombination inEscherichia coli”, Journal of Virology, vol. 70, No. 7, pp. 4805-4810 (Jul. 1996).
Bett et al., “An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3”, Proc. Natl. Acad. Sci. USA, vol. 91, pp. 8802-8806 (Sep. 1994).
Sandig et al., “Optimization of the helper-dependent adenovirus system for production and potencyin vivo”, PNAS, vol. 97, No. 3, pp. 1002-1007 (Feb. 2000).
Grable et al., “Adenovirus Type 5 Packaging Domain is Composed of a Repeat Element that is Functionally Redundant”, Journal of Virology, vol. 64, No. 5, pp. 2047-2056 (May 1990).
Hardy et al., “Construction of Adenovirus Vectors through Cre-lox Recombination”, Journal of Virology, vol. 71, No. 3, pp. 1842-1849 (Mar. 1997).
Ilan et al., “Insertion of the adenoviral E3 region into a recombinant viral vector prevents antivira humoral and cellular immune responses and permits long-term gene expression”, Proc. Natl. Acad. Sci. USA, vol. 94, pp. 2587-2592 (Mar. 1997).
Mittal, et al., “Induction of Systemic and Mucosal Immune Responses in Cotton Rats Immunized with Human Adenovirus Type 5 Recombinants Rxpressing the Full and Truncated Forms of Bovine Herpesvirus Type 1 Glycoprotein gD1”, Virology, vol. 222, pp. 299-309 (1996).
Grable et al., cis and trans Requirements for the Selective Packaging of Adenovirus Type 5 DNA, Journal of Virology, vol. 66, No. 2, pp. 723-731 (Feb. 1992).
Bett Andrew
Sandig Volker
Youil Rima
Giesser J. M.
Hill Myron G.
Housel James
Merck & Co. , Inc.
Switzer J. E.
LandOfFree
Helper dependent vector system for gene therapy does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Helper dependent vector system for gene therapy, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Helper dependent vector system for gene therapy will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3667931