Helper cell

Details Helper cell Helper cell

1984-03-26

1987-03-17

Warren, Charles F.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4351723, 435 91, 435236, 435948, 935 32, 935 71, 935 70, C12N 500, C12N 1500, C12N 704, C12R 191

Patent

active

046507647

ABSTRACT:
A helper cell for providing retrovirus protein which is required by a normally replication incompetent recombinant retrovirus gene sequence in order to replicate is disclosed. In one embodiment there is a eukaryotic host cell, a first retrovirus gene sequence in the cell which has a helper portion coding for a retrovirus protein and which is capable of expressing the retrovirus protein, and a defective portion which renders the gene sequence replication imcompetent. In this embodiment, there is also a second retrovirus gene sequence in the cell having a defective retrovirus port

REFERENCES:
patent: 4399216 (1983-08-01), Axel et al.
patent: 4405712 (1983-09-01), Vande Woulde et al.
Tabin et al, "Adaptation of a Retrovirus as a Eukaryotic Vector Transmitting the HSV Thymidine Kinase Gene", Molecular and Cellular Biology, 2(4) pp. 426-436 (4-1982).
Wei et al, "Construction and Isolation of a Transmissible Retrovirus Containing the Src Gene of Harvey Murine Sarcoma Virus", Journal of Virology, 39(3) pp. 935-944 (1981).
Steimer et al, "Envelope Assembly Mutant of Rouse Sarcoma Virus", Journal of Virology, 36(3), pp. 883-888 (1980).
Robbins et al, "Molecular Cloning of Integrated Simian Sarcoma Virus: Organization of Infectious DNA Clones", Proceedings of the National Academy of Sciences, 78(5) pp. 2918-2922 (1981).
O'Rear et al, "Mapping of Alterations in Noninfectious Proviruses of Spleen Necrosis Virus", Journal of Virology, 39(1) pp. 138-149 (1981).
Linial, "Transfer of Defective Avian Tumor Virus Genomes by Rous Sarcoma Virus RNA Packaging Mutant", Journal of Virology, 38(1) pp. 380-382 (1981).
Watanabe et al, "Encapsidation Sequence Required for Retrovirus Vectors", Eukaryotic Viral Vectors ed. Gluzman, Cold Spring Harbor Lab. 1982 p. 115.
Article by K. Shimotohno & H. Temin, "Formation of Infectious Progeny Virus After Insertion of Herpes Simplex Thymidine Kinase Gene Into DNA of an Avian Retrovirus", 26 Cell 67-77 (1981).
Article by H. Temin, "Structure, Variation and Synthesis of Retrovirus Long Terminal Repeat", 27 Cell 1-3 (1981).
Article by K. Shimotohno & H. Temin, "Loss of Intervening Sequences in Genomic Mouse Alpha Globin DNA Inserted in an Infectious Retrovirus Vector", 299 Nature 265-268 (1982).
Article by S. Watanabe & H. Temin, "Encapsidation Sequences for Spleen Necrosis Virus, An Avian Retrovirus, are Between the 5' Long Terminal Repeat and the Start of the Gag Gene", 79 Proc. Nat'l. Acad. Sci., USA 5986-5990 (1982).
Article by J. O'Rear et al, "Infectious and Noninfectious Recombinant Clones of the Provirus of SNV Differ in Cellular DNA and are Apparently the Same in Viral DNA", 20 Cell 423-430 (1980).
Article by I. Chen et al, "Characterization of Reticuleondotheliosis Virus Stain T DNA and Isolation of a Novel Variant of Reticuleondotheliosis Virus Strain T by Molecular Cloning", 40 J. Virol 800-811 (1981).
Article by P. Southern and P. Berg, "Mammalian Cell Transformation with SV40 Hybrid Plasmid Vectors", Eukaryotic Viral Vectors, pp. 41-45 (Cold Spring Harbor Laboratory 1982).
Article by J. O'Rear and H. Temin, "Spontaneous Changes in Nucleotide Sequence in Provirus of Spleen Necrosis Virus, an Avian Retrovirus", 79 Proc. Nat'l. Acad. Sci., USA pp. 1230-1234 (1982).
Article by Y. Gluzman, "SV40 Transformed Simian Cells Support the Replication of Early SV40 Mutants", 23 Cell 175-182 (1981).

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