Helicobacter pylori antigens in blood

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S006120, C435S091100, C435S091210, C536S023700, C536S024300, C536S024320, C536S024330, C204S166000

Reexamination Certificate

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10922263

ABSTRACT:
The present invention provides methods for detectingHelicobacter pylori(H. pylori) DNA and/or fragments thereof in blood. The first method involves extracting DNA from a blood sample, preferably plasma, by amplifying the DNA using a polymerase chain reaction (PCR) or a ligase chain reaction (LCR) method, and detecting a target DNA sequence in the amplified DNA. The preferred target DNA sequence comprises a Mr26 or a 16S rRNA gene or fragments thereof specific toH. pylori. The second method involves extracting DNA from a blood sample, preferably serum, by hybridizing the extracted DNA with a radioisotope or fluorescence labeledH. pyloriDNA probe.

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