Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-05-26
1998-09-01
Degen, Nancy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 697, 4352523, 4352542, 4353201, 530350, 530 232, 530 234, 530 2432, C12N 1500, C12N 1563, C07K 14195, C07H 2104
Patent
active
058010130
ABSTRACT:
Recombinant nucleic acids which encode aminoacyl-tRNA sythetases of helicobacter origin have been isolated. These nucleic acids can be used to make expression constructs and transformed host cells for the production of helicobacter aminoacyl-tRNA synthetases. They can also be used in the further isolation of nucleic acids related by DNA sequence similarities, which also encode helicobacter aminoacyl-tRNA synthetases, or portions thereof. A further embodiment of the invention is antisense nucleic acid which can hybridize to the nucleic acid which encodes the aminoacyl-tRNA synthetase of helicobacter. The invention also relates to isolated and/or recombinant helicobacter aminoacyl-tRNA synthetases. Antibodies which bind to these enzymes can be made and can be used in the purification and study of the enzymes. Also described are tester strains, which are cells engineered to rely on the function of the tRNA synthetase encoded by an introduced cloned gene, and which can be used to test the effectiveness of drug candidates in the inhibition of an essential aminoacyl-tRNA synthetase encoded by an introduced cloned helicobacter gene.
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Houman Fariba
Qiu Yan
Schimmel Paul R.
Shen Xiaoyu
Tao Jianshi
Cubist Pharmaceuticals, Inc.
Degen Nancy
Larson Thomas G.
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