HEK293 cell line for activity studies and HTS system of...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se

Reexamination Certificate

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C435S325000, C435S069100, C435S006120

Reexamination Certificate

active

06984521

ABSTRACT:
There is provided a cell line that is prepared by transforming HEK293 cell with a human Kir2.1 gene using a retrovirus expression system, wherein the HEK293 expresses a stable α1GT-type calcium channel. The cell line responds sensitively to KCl and forms an appropriate level of the membrane voltage so that the cell signaling pathway may be investigated by the molecular biological and biochemical studies.

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Bernheim et.al., News Physiol. Sci. 17: 22-26,2002.
Picones et.al., Biophysical journal 81:2035-2049, 2001.
Cheng et.al., Blood, 92:83-82, 1998.
Clontechniques, Jan. 1999.

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