Heat-treated IgM antibody preparations

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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5303905, 4241301, 4241771, C07K 1606, A61K 39395

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active

055104657

ABSTRACT:
Mild heat-treatment of IgM antibody concentrates diminishes the potential to induce non-specific complement activation without significant loss of normal immunologic effector functions. These IgM immunoglobulin concentrates retain specific antigen binding properties and activate complement when bound to antigen. Preferred product includes at least 20% by weight IgM in an IgM/IgG antibody mixture. Heating is done at a temperature within the range of about 40.degree. C. to 62.degree. C., preferably about 45.degree. to 55.degree. C., in a solution having an acid pH (preferably 4.0 to 5.0) for at least about 10 minutes.

REFERENCES:
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Haque et al. Am J Dis Child. 142: 1293-1296, 1988.
Wickerhauser et al. Vox Sang 23: 119-125, 1972.
Steinbach et al., Preparative Biochemistry 3: 363-373, 1973.
Barandun, et al., "Intravenous Administration of Human .gamma.-Globulin", Vox. Sang., vol. 7, pp. 157-174 (1962).
Siegel, et al., "Effects of Limited Denaturation by Heat on the Dynamic Conformation of Equine Immunoglobulin M Antibody on the Interaction with Antigens and Complement", Biochemistry, vol. 10, pp. 192-198 (1981).

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