Heat stable debranching enzyme from a Bacillus microorganism

Details Heat stable debranching enzyme from a Bacillus microorganism Heat stable debranching enzyme from a Bacillus microorganism

1993-02-25

1995-02-07

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 98, 4352525, 435832, C12N 944, C12N 120, C12N 100, C12P 1916

Patent

active

053875168

ABSTRACT:
A debranching enzyme, a process for producing the enzyme, a microorganism capable of producing the enzyme, and a process for producing glucose liquids using the enzyme are disclosed. The microorganism is preferrably Bacillus FERM BP-4204, the enzyme is a debranching enzyme which acts on alpha-1,6 glucosidase bonds on produce straight chain amylose, acts on pullulan and also acts on relatively long chain saccharides, wherein said substrate specificity for activity on pullulan is set at 100, the activity of said enzyme on soluble starches relative to said activity on pullulan is from about 20 to 40, the activity of said enzyme on glycogen relative to said activity on pullulan is from about 30 to 60, and the activity of said enzyme on amylopectin relative to said activity on pullulan is from about 10 to 30, has an optimum pH from about 4.5 to 6.0, an optimum temperature from about 60.degree. C. to about 70.degree. C., a molecular weight of about 98 kD as measured by SDS-PAGE, and an isoelectric point of about 2.9. The enzyme can be also used for the production of glucose and maltose.

REFERENCES:
patent: 3804715 (1974-04-01), Sugimoto et al.
patent: 3862005 (1975-01-01), Miyake et al.
patent: 3992261 (1976-11-01), Takasaki et al.
patent: 4011139 (1977-03-01), Horwath et al.
patent: 4902622 (1990-02-01), Nakai et al.

Affiliated with

Also associated with

Rating

Heat stable debranching enzyme from a Bacillus microorganism does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Heat stable debranching enzyme from a Bacillus microorganism, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Heat stable debranching enzyme from a Bacillus microorganism will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1109841