Heat shock regulated production of selected and fused proteins i

Details Heat shock regulated production of selected and fused proteins i Heat shock regulated production of selected and fused proteins i

1983-05-10

1989-01-10

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 4351723, 435255, 435256, 435320, 536 27, 935 28, 935 37, 935 43, 935 47, 935 69, C12P 2100, C12P 2102, C12N 500, C07H 1512

Patent

active

047973595

ABSTRACT:
A process for producing a yeast capable of heat regulated synthesis of selected and fused proteins is disclosed. The process involves the combination of a DNA fragment comprising a heat shock inducible gene of an appropriate yeast microorganism and a second DNA fragment comprising a selected gene with a suitable transfer vector, thereby producing a recombinant transfer vector. A suitable host yeast is contacted with the recombinant transfer vector, cultured and transforments having the desired genetic capability are selected.

REFERENCES:
patent: 4374927 (1983-02-01), Sninsky
Cover pages-Heat Shock, From Bacteriato Man, Cold Spring Harbor Laboratory 1982.
Finkelstein, D. B. and Strausberg, S. (1982), "Expression of a Cloned Yeast Heat Shock Gene," (Abstract handed out to Cold Spring Harbor Conf. attendants on May 5, 1982).
Nasmyth, K. A. and Tatchell, K. (1980), "The Structure of Transposable Yeast Mating Type Loci" Cell, 19:753-764.
Integration, Transcription, and Control of a Drosophila Heat Shock Gene in Mouse Cells; Proc. Natl. Acad. Sci. vol. 78, No. 11, pgs. 7038-7042 by Corces, Pellicer et al.; Nov. 17/81.
Approximate Localization of Sequences Controlling Transcription of a Drosophila Heat-shock Gene; Heat Shock From Bacteria to Man, pp. 27-34, May 5-9, 1982 by Corces, Pellicer et al.
Transcription and Chromatic Structure of Drosophila Heat-shock Genes in Yeast; by Lis, Costlow et al., Heat Shock pp. 57-62, 1982.
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Yeast Genes Fused to B-galactosidase in Escherichia coli Can Be Expressed Normally in Yeast; Proc. Natl. Acad. Sci. vol. 78 No. 4 pp. 2460-2464 by Rose, Casadaban et al.; Apr. '81.
Fusion of Escherichia coli lacZ to the Cytochrome C Gene of Saccharomyces cerecisiae; Proc. Natl. Acad. Sci. vol 78, No. 4 pp. 2199-2203 by Guarente and Ptashne; Apr. '81.
Expression of a Human Gene for Interferon in Yeast; by Hitzeman, Hagie et al.; Oct. 29, 1981 Nature vol. 293, pp. 717-722.
Synthesis and Assembly of Hepatitis B Virus Surface Antigen Particles in Yeast; Valenzuela, Medina et al.; Nature vol. 298, pp. 347-350. Jul. 22, 1982.

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