Heat shock process for the isolation of bacterial protein

Details Heat shock process for the isolation of bacterial protein Heat shock process for the isolation of bacterial protein

1983-03-09

1984-09-18

Schain, Howard E.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

424 92, C07G 700, A61K 3902, A61K 3574

Patent

active

044723024

ABSTRACT:
There is disclosed an improved process for the isolation of K-99 outer membrane protein pili from Escherichia coli cells. The improved process involves a heat shock treatment of the E. coli cells in a buffered solution to afford a release of the K-99 pili into solution which on standing results in the aggregation and precipitation of the pili. The thus isolated K-99 protein pili is useful in the preparation of vaccines.

REFERENCES:
patent: 3718541 (1973-02-01), Kalina
J. of General Microbiology (1977), 99, 353-357, Morris et al.
Isaacson, Infection and Immunity, 15, pp. 272-279, (1977).
DeGraff et al., Infection and Immunity, 33, pp. 877-883, (1981).
Altmann et al., Biochemistry Journal, 201, pp. 505-513, (1982).
Korhonen et al., Infection and Immunity, 27, pp. 569-575, (1980).
Klemm, European Journal of Biochemistry, 117, pp. 617-627, (1981).
Evans et al., Infection and Immunity, 19, pp. 727-736, (1978).
Fader et al., The Journal of Biological Chemistry, 257, pp. 3301-3305, (1982).

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