Heat-inducible promoter

Details Heat-inducible promoter Heat-inducible promoter

2005-02-08

2005-02-08

Leffers, Gerry (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C536S023100, C536S023400, C536S024100, C435S071100, C435S320100, C435S471000, C435S070100, C435S254200, C435S254110

Reexamination Certificate

active

06852511

ABSTRACT:
The invention relates to nucleic acid molecules comprising a heat-inducible promoter and to expression vectors and host cells containing at least one nucleic acid molecule according to the invention. The present invention further relates to kits and methods for producing one or more proteins using the nucleic acid molecules according to the invention and to various uses of the same. The object of the invention is to provide a promoter the heat-inducible characteristic of which is as selective as possible, in particular a promoter which is active in yeasts and which is suitable for protein expression at high temperatures. This object is fulfilled by a nucleic acid molecule comprising a heat-inducible promoter of aHansenula polymorphagene coding for a protein with trehalose-6-phosphate-synthase activity;

REFERENCES:
patent: 5792921 (1998-08-01), Londesborough et al.
Xia-Dong et al. Conservation of a stress response: human heat shock transcription factors functionally substitute for yeast HSF, Nov. 1, 1997. EMBO, 16(21): 6466-6477.*
Blazquez, Miguel A. et al., “Trehalose-6-P synthase is dispersable for growth on glucose but not for spore germination inSchizosaccharomyces pombe”, J. Bacteriol. (1994), 176(13), 3895-3902.
Reinders, Anke et al., “The thermophilic yeastHansenula polymorphadoes not require trehalose synthesis for growth at high temperatures but does for normal acquisition of thermotolerance”, J. Bacteriol. (1999), 181(15), 4665-4668.
Romano, I. “Hansenula polymorphaTPS1 gene”, EMBL Database: Accession No. AJ010725, Mar. 2, 1999.

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