Heat-inducible N-degron module

Details Heat-inducible N-degron module Heat-inducible N-degron module

1994-02-04

1996-07-23

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 6, 435 697, 435189, 530402, 536 221, 536 231, 536 232, 536 234, 536 235, C12P 2106, C12Q 168, C07H 1900, C07H 2100

Patent

active

055388620

ABSTRACT:
The N-degron is an intracellular degradation signal whose essential determinant is a specific, destabilizing, N-terminal amino acid residue. A set of N-degrons containing different destabilizing residues is manifested as the N-end rule, which relates the in vivo half-life of a protein to the identity of its N-terminal amino acid residue. Disclosed herein is a heat-inducible N-degron module. A heat-inducible N-degron module is a protein or peptide bearing a destabilizing N-terminal amino acid residue which becomes a substrate of the N-end rule pathway only at a temperature high enough to result in at least partial unfolding of the protein. At this elevated (nonpermissive) temperature, the heat-inducible N-degron module (and any protein or peptide attached at its C-terminus) is rapidly degraded in a cell in which the N-end rule pathway is operative. Also disclosed are DNA and protein fusion constructs, methods for screening for additional heat-inducible N-degron modules and methods for using the disclosed heat-inducible N-degron modules.

REFERENCES:
patent: 5093242 (1992-03-01), Bachmair et al.
patent: 5122463 (1992-06-01), Varshavsky et al.
patent: 5132213 (1992-07-01), Bachmair et al.
patent: 5196321 (1993-03-01), Bachmair et al.
patent: 5212058 (1993-05-01), Baker et al.
Park, E-C., et al., "A Strategy for the generation of conditional mutations by protein destabilization", Proc. Natl. Acad. Sci. USA, 89:1249-1252 (1992).
Varshavsky, A., "The N-End Rule", Cell, 69:725-735 (1992).
Pringle, J. R., "Induction, Selection, and Experimental Uses of Temperature-Sensitive and Other Conditional Mutants of Yeast", in Methods in Cell Biology, vol. 12 (Prescott ed.), pp. 233-273, Academic Press, New York (1975).

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