Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1979-11-13
1981-08-18
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 96, 435 99, 435202, 435832, 426 48, C12N 928, C12P 1914, C12P 1924, C12P 1920
Patent
active
042847222
ABSTRACT:
Heat and acid-stable alpha-amylase enzymes having the following characteristics: (1) capable of retaining at least about 70% of their initial activity when held at 90.degree. C. and at a pH of 6.0 for 10 minutes in the absence of calcium ion; (2) capable of retaining at least about 50% of their initial activity when held at 90.degree. C. at a pH of 6.0 for 60 minutes in the absence of added calcium ion; and/or (3) capable of retaining at least about 50% of their initial activity at a temperature of 80.degree. C. and at a pH of 4.5 in the presence of 5 mM calcium ion for 10 minutes. The preferred alpha-amylases are prepared by culturing a strain of a Bacillus stearothermophilus microorganism in a suitable culture medium. The novel alpha-amylases are useful in hydrolyzing and/or liquefying starch and due to their stability at low pH values they can be used in conjunction with other acid stable amylases such as gluco-amylase in either a soluble or an immobilized form.
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Ishii Yoshiko
Kanno Mitsuo
Tamuri Masaki
CPC International Inc.
Wiseman Thomas G.
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