HCV replicase complexes

Details HCV replicase complexes HCV replicase complexes

2005-11-22

2005-11-22

Scheiner, Laurie (Department: 1648)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

C435S235100

Reexamination Certificate

active

06967075

ABSTRACT:
This invention provides efficient HCV replicase complexes comprising novel RNA template and primer pair. Assay systems are also provided, which use such complexes, for detecting replicase activity, quantitatively studying the kinetics and mechanism of HCV NS5B-catalyzed nucleotide incorporation, and identifying inhibitors of HCV replicase. The assay systems use small and well-defined synthetic RNAs which allow efficient assembly of all catalytic components in the quaternary complex for HCV NS5B-directed RNA replication. Specific template-primer requirements for efficient RNA synthesis by HCV NS5B replicase are provided for use in assay systems.

REFERENCES:
Ago et al., Structure 7: 1417-1426 (1999).
Al et al., Virus Res. 53: 141-149 (1998).
Arnold and Cameron, J. Biol. Chem. 274: 2706-2716 (1999).
Arnold and Cameron, J. Biol. Chem. 275: 5329-5336 (2000).
Bressanelli et al., Proc. Natl. Acad. Sci USA 96:13034-13039 (1999).
Behrens et al., EMBO J. 15: 12-22 (1996).
Carpousis and Gralla, Biochemistry 19: 3245-3253 (1980).
Cheetham & Steitz, Science 286: 2305-2309 (1999).
De Francesco et al., Methods Enzymol. 275: 58-67 (1996).
Ferrari et al., J. Virol. 73: 1649-54 (1999).
Joyce and Steitz, Annu. Rev. Biochem. 63: 777-822 (1997).
Kao et al., Virology 253: 1-7 (1999).
Kuo et al., Science 244: 362-364 (1989).
Lai et al., J. Virol. 73: 10129-10136 (1999).
Lesburg et al., Nat. Struct. Biol. 6: 937-943 (1999).
Lohmann et al., J. Virol. 71: 8416-8428 (1997).
Lohmann et al., Virol. 249: 108-118 (1998).
Love et al., Cell 87: 331-342 (1996).
Luo et al., J. Virol. 74: 851-863 (2000).
Oh et al., J. Virol. 73: 7694-7702 (1999).
Paul et al., Nature 393:280-284 (1998).
Plotch et al., J. Virol. 63: 216-225 (1989).
Yuan et al., Biochem. Biophys. Res. Commun. 232: 231-235 (1997).
Zhong et al., J. Virol. 72:9365-9369 (1998).
Zhong et al., J. Virol. 74: 2017-2022 (2000).

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