HCV NS3 protein fragments having helicase activity and improved

Details HCV NS3 protein fragments having helicase activity and improved HCV NS3 protein fragments having helicase activity and improved

1997-04-08

1999-11-23

Achutamurthy, Ponnathapura

Chemistry: molecular biology and microbiology

Vector, per se

536 232, 536 234, 536 2372, C12N 1500, C07H 2104

Patent

active

059899050

ABSTRACT:
The Hepatitis C Virus (HCV) NS3 protein contains amino acid motifs of a serine proteinase, a nucleotide triphosphatase (NTPase), and an RNA helicase. A carboxy fragment of the HCV NS3 protein was purified and possessed RNA helicase activity. Detections from the amino terminus resulted in the protein becoming soluble. Deletions from the carboxy terminus do not result in a loss of helicase activity until at least 50 amino acids are deleted. The helicase activity requires ATP and divalent cations such as Mg.sup.2+ and Mn.sup.2+. The helicase activity was blocked by monoclonal antibody specific to the HCV NS3 protein.

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