Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...
Reexamination Certificate
2006-04-25
2006-04-25
Slobodyansky, Elizabeth (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
C435S193000, C536S023200
Reexamination Certificate
active
07033805
ABSTRACT:
The Hepatitis C Virus (HCV) NS3 protein contains amino acid motifs of a serine proteinase, a nucleotide triphosphatase (NTPase), and an RNA helicase. A carboxy fragment of the HCV NS3 protein was purified and possessed RNA helicase activity. Detections from the amino terminus resulted in the protein becoming soluble. Deletions from the carboxy terminus do not result in a loss of helicase activity until at least 50 amino acids are deleted. The helicase activity requires ATP and divalent cations such as Mg2+and Mn2+. The helicase activity was blocked by monoclonal antibody specific to the HCV NS3 protein.
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Choe Joonho
Choo Qui-Lim
Han Jang
Houghton Michael
Chiron Corporation
Harbin Alisa A.
Robins Roberta L.
Slobodyansky Elizabeth
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