HCG peptides for use in antibody purification procedures

Details HCG peptides for use in antibody purification procedures HCG peptides for use in antibody purification procedures

1991-01-30

1995-09-19

Jones, W. Gary

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436547, 436807, 436824, 530324, 5303911, 530399, 530403, 530413, G01N 33543, G01N 3353, A61K 3702, C07K 300

Patent

active

054515270

ABSTRACT:
An affinity column antibody purification method using a synthetic hCG analyte-analog insolubilized on a support material. The immobilized hCG analyte-analogs are peptide sequences which duplicate or mimic the determinants formed by the amino acid residues of the C-terminus peptide sequence or portions or variants thereof. The antibodies produced according to the present invention are useful in immunoassays for hCG.

REFERENCES:
Bidart et al. Mol. Immunol. 24(4). 1987 339-346 (abstract only).
Oztark et al. Endocrinology 120 (2) 1987 559-566 (abstract only).
Story et al. J. Clin. Endrcrinol. Metab. 53 (5) 1981 1090-1095 (abstract only).
Emlen et al. J. Immunol Methods 62 (2) 1983 205-216 (abstract only).
Computer generated sequence comparison of .beta.-hcG.
Gaysen et al. P.N.A.S. U.S.A. vol. 81, pp. 3998-4002, Jul. 1984.
Fiddes et al., Nature, vol. 286, 14 Aug. 1980, pp. 684-687.
Birken et al. "The Journal of Biological Chemistry", vol. 256, No. 4, 25 Feb. 1981, pp. 1816-1823.

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