HBV polymerase, RNase H enzyme derived from HBV polymerase, proc

Details HBV polymerase, RNase H enzyme derived from HBV polymerase, proc HBV polymerase, RNase H enzyme derived from HBV polymerase, proc

1999-01-11

2000-06-06

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 697, 4352523, 43525411, 4353201, 435325, 435410, C12N 922, C12N 120, C12N 1500, C12N 500, C12P 2104

Patent

active

060717343

ABSTRACT:
The present invention relates to hepatitis B virus (hereinafter it refers to HBV) polymerase containing a histidine tag, RNase H enzyme derived from HBV polymerase and processes for preparation thereof. More particularly, the present invention relates to recombinant HBV polymerase, its RNase H domain with enzyme activity, expression vectors producing the enzymes in E. coli and processes for preparing the HBV polymerase and the RNase H enzyme which can be easily purified due to their histidine tags. And the present invention relates to uses of the HBV polymerase and the RNase H enzyme for screening antiviral agents.

REFERENCES:
Muller et al., "Co-expression of the Subunits of Heterodimer of HIV-1 Reverse Transcriptase in Escherichia coli", J. Bio. Chem 264, No. 24, pp. 13975-13976 (Aug. 1989).
Tan et al., "Inhibition of the RNase H Activity of HIV Reverse Transcriptase by Azidothymidylate", Biochemistry 30, No. 20, pp. 4831-4835 (May 1991).
Smith et al., "Purification and Characterization of Active Human Immunodeficiency Virus Type 1 RNase H Domain", Journal of Virology 67, No. 7, pp. 4037-4049 (Jul. 1993).

Affiliated with

Also associated with

Rating

HBV polymerase, RNase H enzyme derived from HBV polymerase, proc does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with HBV polymerase, RNase H enzyme derived from HBV polymerase, proc, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and HBV polymerase, RNase H enzyme derived from HBV polymerase, proc will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2212193