Harmonic generation microscopy

Optical: systems and elements – Optical frequency converter – Harmonic generator

Reexamination Certificate

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C359S328000, C359S381000, C356S904000

Reexamination Certificate

active

06922279

ABSTRACT:
A harmonic generation microscopy employs a laser device that emits a laser beam having a predetermined wavelength that causes no autofluorescence in a biological sample and that, after excited, induces both the second and third harmonic waves. The laser beam is projected onto a sample and an observation beam from the sample is received. The observation beam is directed through a splitter to separate the second harmonic wave and the third harmonic wave both of which are then converted into corresponding electrical signals. The electrical signals are fed to a computer-based image processing equipment to form an image of the sample on the basis of the second and third harmonic waves.

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Nonlinear scanning laser microscopy by third harmonic generation Y. Barad, H. Eisenberg, M. Horowitz and Y. Silberberg, Applied Physics Letters, 1997, vol. 70, pp 922-924.

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