Haptens tracers, immunogens and antibodies for 3-phenyl-1-adaman

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic

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435 6, 436546, 436822, 5303889, 5303898, 530404, 530405, 530406, 530807, G01N 33536, G01N 33577, C07K 1644

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active

056165056

ABSTRACT:
Novel tethered hapten intermediates and related conjugates based on 3-phenyl-1-adamantaneacetic acid, as well as methods for making and using such conjugates. Haptens based on the above core structure may be substituted at any position on the phenyl ring, especially at the para position. Using tethered intermediates, immunogens, tracers, solid supports and labeled oligonucleotides are all described; as are methods for using the intermediates to prepare the conjugates, methods of using the conjugates to make and purify anitbodies, as assay tracers, and in nucleic acid hybridization assays. Kits containing haptenated oligonucleotides and anti-hapten conjugates are also described.

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Danilenko, et al., Synthesis and Biological Activity of Adamantane Derivatives, V. Virus-Inhibiting Effect of Arylamides of Adamantanecarboxylic Acids, Khim.-Farm. Zh., 10(7):60-62 (1976) (Russian Paper Corresponding to CA86(17):120855e).
Kohler, et al., Continuous cultures of fused cells secreting antibody of prediefined specificity. Nature, vol. 256, (1975) pp. 495-497.
Matthews, et al., Analytical Strategies for the Use of DNA Probes, Analytical Biochemistry, vol. 169, (1988) pp. 1-25.
Fidanza, et al., Use of a Thiol Tether for the Site-Specific Attachment of Reporter Groups to DNA J. Org. Chem., vol. 57, (1992), pp. 2340-2346.
McConnell, et al., Cell Interactions in the Immune Response The Immune System--A Course on the Molecular and Cellular Basis of Immunity Chapter 10, pp. 158-159.

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