Hapten-inhibitor immunoassay

Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal

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435 7, 435184, 260112T, 436500, G01N 3154, C12N 999, C07G 700

Patent

active

044302630

ABSTRACT:
A specific binding assay method employing, as a labeling substance, a reversible trypsin inhibitor for the detection of a hapten. Competition between the hapten to be determined and hapten trysin inhibitor conjugate for antibody to the hapten, in the presence of enzyme, followed by addition of enzyme substrate provides an effective method for hapten analysis. The preferred trypsin inhibitor is a protein having a molecular weight range of 2,000-75,000. The preferred ratio of the hapten to the inhibitor in the conjugate is between 1:1 and 3:1.

REFERENCES:
patent: 3049530 (1962-08-01), Rackis et al.
patent: 3912704 (1975-10-01), Singh
patent: 3957342 (1976-08-01), Gross
patent: 3983099 (1976-09-01), Niswender
patent: 4043872 (1977-08-01), Blakemore et al.
patent: 4065354 (1977-12-01), Ullman et al.
patent: 4130462 (1978-12-01), Rubenstein et al.
patent: 4134792 (1979-11-01), Boguslaski et al.
Derwent, "Natural Products," p. 33 Week A26, Mile-46105A/26 DT2754-26.
Schroeder, et al., "Specific Binding Reactions Monitored with Ligand-Cofactor Conjugates and Bacterial Luciferase", Anal. Biochem., vol. 72 (1976), pp. 283-292.
Carrico, et al., "A Method for Monitoring Specific Binding Reactions with Cofactor Labeled Ligands", Anal. Biochem., vol. 72 (1976), pp. 271-282.

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