Hapten derivatized capture membrane and diagnostic assays using

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435442, 436501, 436530, 436532, 436543, 436 63, 436823, 536 221, 536 2532, C12Q 168, G01N 33566, C07H 1900, C07H 2100

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058887288

ABSTRACT:
The present invention encompasses a capture membrane comprising a porous filter membrane having a hapten bound directly or indirectly to the membrane wherein complexes formed by specific binding having an anti-hapten bound to a binding member of the specifically binding complex are removed from a solution by the hapten as the solution passes through the membrane. In the preferred embodiment biotin is the hapten and avidin or streptavidin is the anti-hapten.

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Wilchek et al., "Review. The avidin-biotin complex in bioanalytical applications" Analytical Biochemistrry, vol. 171, pp. 1-32 1988.
Syvanen et al. "Fast quantification of nucleic acid hybrids by affinity-based hybrid collection" Nucleic Acids Research, vol. 14 No. 12, pp. 5037-5048, 1986.
Chelly et al. "Transcription of the dystrophin gene in human muscle and non-muscle tissues". Nature, vol. 333, pp. 858-860, 1988.

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