Hansenula polymorpha gene coding for α...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S193000, C435S015000, C435S254230, C435S254110, C435S320100, C435S072000, C435S101000, C536S023100, C536S023200, C530S395000

Reexamination Certificate

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07867730

ABSTRACT:
Disclosed are a novelHansenula polymorphagene coding for α-1,6-mannosyltransferase initiating outer chain elongation, anH. polymorphamutant strain having a deficiency in the gene, and a process for producing a recombinant glycoprotein using such a mutant strain.

REFERENCES:
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patent: 2007150780 (2007-06-01), None
patent: WO 92/19741 (1992-11-01), None
patent: WO 02/00856 (2002-01-01), None
patent: WO 02/00879 (2002-01-01), None
patent: WO 2004/003205 (2004-01-01), None
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Kim et al., Functional characterization of theHansenula polymorphaHOC1 . . . J. Biol. Chem., 2006, vol. 281 (10): 6261-6272.
English Translation of Abstract; JP Patent Publication No. JP2007150780; Published: Jun. 14, 2007; Applicant: CB KK; (1 pg.).
Chiba, Yasunori, et al.; Production of Human Compatible High Mannose-type (Man5GlcNAc2) Sugar Chains inSaccharomyces cerevisiae; The Journal of Biological Chemistry (Oct. 9, 1998); vol. 273, No. 41, pp. 26298-26304.
Ballou, Clinton; “Isolation, Characterization, and Properties ofSaccharomyces cerevisiaemnn Mutants with Noncondictional Protein Glycosylation Defects”; Methods Enzymol. (1990); pp. 440-441.
Choi, Byung-Kwon, et al.; Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeastPichia pastoris; PNAS (Apr. 29, 2003); vol. 100, No. 9; pp. 5022-5027.
Nakanishi-Shindo, Yoko, et al.; Structure of the N-Linked Oligosaccharides That Show the Complete Loss of a-1,6-Polymannose Outer Chain from och1, och1, mnn1, and och1 mnn1 alg3 Mutants ofSaccharomyces cerevisiae; The Journal of Biological Chemistry (Dec. 15, 1993); vol. 268, No. 35; pp. 26338-26345.

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