Chemical apparatus and process disinfecting – deodorizing – preser – Physical type apparatus – Crystallizer
Patent
1988-03-23
1989-12-12
Doll, John
Chemical apparatus and process disinfecting, deodorizing, preser
Physical type apparatus
Crystallizer
156DIG62, 156DIG72, 156608, 156600, C30B 702
Patent
active
048866466
ABSTRACT:
An apparatus (10) is constructed having a cylindrical enclosure (16) within which a disc-shaped wicking element (18) is positioned. A well or recess (22) is cut into an upper side (24) of this wicking element, and a glass cover plate or slip (28) having a protein drop disposed thereon is sealably positioned on the wicking element (18), with drop (12) being positioned over well or recess (22). A flow of control fluid is generated by a programmable gradient former (16), with this control fluid having a vapor pressure that is selectively variable. This flow of control fluid is coupled to the wicking element (18) where control fluid vapor diffusing from walls (26) of the recess (22) is exposed to the drop (12), forming a vapor pressure gradient between the drop (12) and the control fluid vapor. Initially, this gradient is adjusted to draw solvent from the drop (12) at a relatively high rate, and as the critical supersaturation point is approached (the point at which crystal nucleation occurs), the gradient is reduced to more slowly draw solvent from the drop (12). This allows discrete protein molecules more time to orient themselves into an ordered crystalline lattice, producing protein crystals which, when processed by X-ray crystallography, possess a high degree of resolution.
REFERENCES:
patent: 4263010 (1981-04-01), Randolph
patent: 4755363 (1988-07-01), Fujita et al.
Carter Daniel C.
Smith Robbie E.
Doll John
Franklin M.
Glenn Charles E. B.
Manning John R.
Sheehan William J.
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