Hammerhead ribozymes with enhanced stability provided by an addi

Details Hammerhead ribozymes with enhanced stability provided by an addi Hammerhead ribozymes with enhanced stability provided by an addi

1993-08-24

1995-07-25

Stone, Jacqueline

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 231, 536 245, 435 6, 435 9121, 435 9131, 4351723, 4353201, C12N 922, C12N 1566

Patent

active

054363306

ABSTRACT:
A DNA fragment is disclosed which has connected to the downstream side of a first DNA sequence containing a region coding for the first ribozyme RNA a second DNA sequence containing a region coding for second ribozyme RNA capable of cleaving by self-processing the 3'-terminus site of the first ribozyme RNA subjected to transcription. A ribozyme having the 3'-terminus site thereof self-processed is obtained by effecting transcription of a RNA using as a template a recombinant vector obtained by recombination by the use of the DNA fragment.

REFERENCES:
Dzianott et al. (1989), Proc. Natl. Acad. Sci., vol. 86, pp. 4823-4827.

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