Haloalkyl derivatives of reporter molecules used to analyze meta

Details Haloalkyl derivatives of reporter molecules used to analyze meta Haloalkyl derivatives of reporter molecules used to analyze meta

1994-11-08

1996-11-19

Peselev, Elli

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 41, 536 181, 549356, 549382, C07H 1700, C07D30900

Patent

active

055764240

ABSTRACT:
The subject invention provides substrates useful for analyzing the metabolic activity in cells by improving the retention of a detectable reporter molecule only in intact cells where a particular enzyme is present. In particular, improved retention results from a two part process involving conjugation of haloalkyl-substituted derivatives of a reporter molecule with intracellular cysteine-containing peptides while unblocking the reporter molecule. The substrates have the form

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