Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-02-03
1995-11-14
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 23, 435 24, 435961, 435962, 435693, 436175, 436177, 436518, 436815, 436825, 436901, G01N 3353
Patent
active
054665795
ABSTRACT:
A method for the direct analysis of analyte indicative of marijuana exposure found in keratinized structures, e.g., hair, fingernails and toenails, which comprises preparing a mixture containing dithiothreitol or dithioerythritol, a protease suitable for the digestion of the keratin structure and a sample of the keratin structure; permitting the enzyme to at least substantially digest the sample of keratin structure to form a digest solution, followed by mixing the digest solution with a suspension of an ion exchange resin to remove an interfering, cross reacting substance naturally found in hair and finally subjecting the digest solution to analysis to determine the identity and amount of marijuana analyte in the keratin structure sample. To accelerate the method, cupric sulfate may be added to the mixture after degradation of the keratin sample in order to deactivate the activator. The enzyme may be a protease with papain, chymopapain, and proteinase K being preferred for use in the invention. Exemplary ion exchange resins useful in the method according to the invention are DEAE Sephadex (Diethylaminoethyl Sephadex) and DEAE Sepharose (Diethylaminoethyl Sepharose).
REFERENCES:
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Julesz et al., "Steroids in Human Skin and Hairs, IV. Neutral. 17-Keto-Steroids in Human Hairs", Acta Medica Academiae Scientarum Hungaricae Tomus, vol. 22, No. 1, pp. 49-52, (1966).
Rodgers et al., "Homogeneous Enzyme Immunoassay for Cannabinoids in Urine", Clin. Chem., vol. 24, No. 1, pp. 95-100, (1978).
Axelson et al., "Analysis of Profiles of Conjugated Steroids in Urine by Ion-Exchange Separation and Gas Chromotography-Mass Spectroscopy", J. Chrom., vol. 224, pp. 355-370, (1981).
Paul et al., "Detection and Quantitation of Urinary II-Nor-Delta-9-Tetrahydro-Cannabinol-9-Carboxylic Acid, A Metabolite of Tetrahydrocannabinol, by Cappilary Gas Chromotography and Electron Impact Mass Fragmentography", J. Analyt. Toxicol., vol. 11, No. 1, Abstract, (1987).
Badke Bradford J.
Psychemedics Corporation
Scheiner Toni R.
Wolski Susan C.
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