H. influenzae HxuB and HxuC genes, proteins and methods of use

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4352523, 43525233, 435 693, 536 237, C12P 2106, C12N 120, C07H 2104

Patent

active

060201541

ABSTRACT:
Disclosed are hxuB and hxuC genes and DNA segments from H. influenzae such as H. influenzae type b (Hib) and nontypeable H. influenzae (NTHI). The HxuB and HxuC proteins, being surface expressed, are contemplated for use in the preparation of vaccines against pathological H. influenzae infections, and in particular, vaccines for use in children and other individuals who respond poorly to capsular-based vaccines. DNA segments encoding these proteins and anti- HxuB and HxuC antibodies will also be of in various screening, diagnostic and therapeutic applications.

REFERENCES:
patent: 4668624 (1987-05-01), Roberts
patent: 5362865 (1994-11-01), Austin
patent: 5378619 (1995-01-01), Rogers
patent: 5380655 (1995-01-01), Hansen
Database Genbank, Accession No. U09840, submitted May 18, 1994 by Cope et al., available Dec. 7, 1994.
Alberts, B. et al. `Molecular Biology of the Cell,` Second Edition, published 1989, by Garland Publishing (New York), pp. 262-266, 1989.
Barenkamp and Geme, "Genes Encoding High-Molecular-Weight Adhesion Proteins of Nontypeable Haemophilus influenzae Are Part of Gene Clusters," Infection and Immunity, 62(8):3320-3328, Aug. 1994.
Braun et al., "Serratia marcescens forms a new type of cytolysin," FEMS Microbiol. Lett, 100:299-306, 1992.
Cope et al., "The 100 kDa Heme:Hemopexin-Binding Protein of Haemophilus influenzae is Present in Soluble Form in Culture Supernatant," Abstracts of the 94th Gen. Meeting of the Am. Soc. for Microbiol., 1994.
Cope et al., "The 100 kDa haem:haemopexin-binding protein of Haemophilus influenzae: structure and localization," Mol. Microbiol., 13:863-873, 1994.
Gulig et al., "Immunogenic proteins in cell-free culture supernatants of Haemophilus influenzae type b," Infect. Immun., 44:41-48, 1984.
Hannavy and Higgins, "TonB; a model for signal transduction between membranes," Biochem. Soc. Transact., 19:530-532, 1991.
Hanson and Hansen, "Molecular cloning, partial purification, and characterization of a haemin-binding lipoprotein from Haemophilus influenzae type b," Mol. Microbiol., 5:267-278, 1991.
Hanson et al., "Identification of a genetic locus of Haemophilus influenzae type b necessary for the binding and utilization of heme bound to human hemopexin," Proc. Natl. Acad. Sci. USA, 89:1973-1977, 1992.
Heller and Kadner, "Nucleotide sequence of the gene for the vitamin B.sub.12 receptor protein in the outer membrane of Escherichia coli," J. Bacteriol., 161:904-908, 1985.
Koebnik et al., "The TonB protein of Yersinia enterocolitica and its interactions with TonB-box proteins," Mol. Gen. Genet., 237:152-160, 1993.
Jareosik et al., "A functional tonB gene is required for both utilization of heme and virulence expression by Haemophilus influenzae type B," Infect. Immun, 62:2470-2477, 1994.
Lee, "Isolation of an outer membrane hemin-binding protein of Haemophilus influenzae type b," Infect. Immun, 60:810-816, 1992.
Litwin and Calderwood, "Role of iron in regulation of virulence genes," Clin. Microbiol. Rev., 6:137-149, 1993.
Lundrigan and Kadner, "Nucleotide sequence of the gene for the ferrienterochelin receptor FepA in Escherichia coli: homology among outer membrane receptors that interact with TonB," J. Biol. Chem, 261:10797-10801, 1986.
Nau and Konisky, "Evolutionary relationship between the TonB-dependent outer membrane transport proteins: nucleotide and amino acid sequences of the Escherichia coli colicin I receptor gene," J. Bacteriol., 171:1041-1047, 1989.
Plaut et al., "Growth of Haemophilus influenzae in human milk: Synthesis, distribution, and activity of IgA protease as determined by study of iga+ and mutant iga- cells," J. Infect Dis, 166:43-52, 1992.
Poole et al., "Molecular characterization of the hemolysin determinant of Serratia marcescens," J. Bacteriol., 170:3177-3188, 1988.
Postle, "TonB and the Gram-negative dilemma," Mol. Microbiol., 4:2019-2025, 1990.
Schiebel et al., "Subcellular location and unique secretion of the hemolysin of Serratia marcescens," J. Biol. Chem., 264:16311-16320, 1989.
Sjostrom et al., "Signal peptide amino acid sequences in Escherichia coli contain information related to final protein localization: A multivariate data analysis," EMBO J., 6:823-831, 1987.
Stojiljkovic and Hantke, "Hemin uptake system of Yersinia enterocolitica: similarities with other TonB-dependent systems in Gram-negative bacteria," EMBO J., 11:4359-4367, 1992.
Stull, "Protein sources of heme for Haemophilus influenzae," Infect. Immun., 55:148-153, 1987.
Uphoff and Welch, "Nucleotide sequencing of the Proteus mirabilis calcium-independent hemolysin genes (hmpA and hpmB) reveals sequence similarity with the Serratia marcescens hemolysin genes (sh1A and sh1B),"J. Bacteriol., 172:1206-1216, 1990.
von Heijne, G., "Signal sequences: The limits of variation," J. Mol. Biol., 184:99-105, 1985.
Welch, "Pore-forming cytolysins of Gram-negative bacteria," Mol. Microbiol., 5:521-528, 1991.
Wong et al., "Identification and characterization of an iron-regulated hemopexin receptor in Haemophilus influenzae type b," Infect. Immun., 62:48-59, 1994.
Wong et al., "Affinity, Conservation, and Surface Exposure of Hemopexin-Binding Proteins in Haemophilus influenzae," Infection and Immunity, 63(6):2327-2333, Jun. 1995.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

H. influenzae HxuB and HxuC genes, proteins and methods of use does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with H. influenzae HxuB and HxuC genes, proteins and methods of use, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and H. influenzae HxuB and HxuC genes, proteins and methods of use will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-936422

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.