Guanylate binding proteins

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4353201, 4352521, 536 232, C07H 2104, C12N 170, C12N 1563

Patent

active

058719652

ABSTRACT:
The present invention provides two human guanylate binding proteins (designated collectively as HGBP) and polynucleotides which identify and encode HGBP. The invention also provides genetically engineered expression vectors and host cells comprising the nucleic acid sequences encoding HGBP and a method for producing HGBP. The invention also provides for use of HGBP and agonists, antibodies, or antagonists specifically binding HGBP, in the prevention and treatment of diseases associated with expression of HGBP. Additionally, the invention provides for the use of antisense molecules to polynucleotides encoding HGBP for the treatment of diseases associated with the expression of HGBP. The invention also provides diagnostic assays which utilize the polynucleotide, or fragments or the complement thereof, and antibodies specifically binding HGBP.

REFERENCES:
EMBL Sequence Database, Heidelberg, DE., Accession NR. AA075671, Hillier, L., et al., "Clone 545038 Similar to GB:M55543 Interferon-Induced Guanylate-Binding Proteing (Human)", XP002057290 Oct. 9, 1996.
Cheng, Y.E., et al., "Affinity Purification of an Interferon-induced Human Guanylate-binding Protein and Its Characterization," The Journal of Biological Chemistry, 260(29) :15834-15839 (1985).
Hamm, H.E., et al., "Site of G Protein Binding to Rhodopsin Mapped with Synthetic Peptides from the .alpha. Subunit," Science, 241:832-834 (1988).
The Pharmacological Basis of Therapeutics, 50:1211-1215 (1996).
Wynn, T.A., et al., "Identification and Characterization of a New Gene Family Induced During Macrophage Activation" J. Immunol., 147:4384-4392 (1991).
Cheng, Y.S.E., et al., "Interferon-Induced Guanylate-Binding Proteins Lack an N(T)KXD Consensus Motif and Bind GMP in Addition to GDP and GTP" Mol. Cell. Biol., 11:4717-4725 (1991).
Schwemmle, M., et al., "The Interferon-induced 67-kDa Guanylate-binding Protein (hGBP1) Is a GTPase That Converts GTP to GMP" J. Biol. Chem., 269:11299-11305 (1994).
Neun, R., et al., "GTPase properties of the interferon-induced human guanylate-binding protein 2" FEBS Letters, 390:69-72 (1996).
Vestal, D.J., et al., "Rat p67 GBP is Induced by Interferon-.gamma. and Isoprenoid-Modified in Macrophages" Biochem. Biophys. Res. Commun., 224:528-534 (1996).

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