Guanosine derivatives and use thereof

Details Guanosine derivatives and use thereof Guanosine derivatives and use thereof

2004-02-23

2009-06-23

McIntosh, III, Traviss C (Department: 1623)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S026700

Reexamination Certificate

active

07550581

ABSTRACT:
It is intended to provide a novel monomer unit by which Z type DNA can be more effectively stabilized, a reagent for integrating this monomer unit into an oligonucleotide, and a method of stabilizing Z type DNA by using the reagent. Namely, a guanosine derivative represented by the following general formula [1]:wherein R1represents acyl; R2represents lower alkyl; R3represents tri-substituted silyloxy or tetrahedropyranyloxy; and R4represents cyanoethyl or allyl; a reagent for stabilizing Z type DNA which contains the guanosine derivative; and a method of stabilizing Z type DNA by using the guanosine derivative. It is also intended to provide a method of transferring guanosine having lower alkyl at the 8-position into an oligonucleotide by using the guanosine derivative; and an oligonucleotide carrying guanosine having lower alkyl at the 8-position transferred thereinto.

REFERENCES:
patent: 4415732 (1983-11-01), Caruthers et al.
patent: 5292873 (1994-03-01), Rokita et al.
Uesugi et al., “Ribooligonucleotides, r(C-G-C-G) Analogues Containing 8-Substituted Guanosine Residues, Form Left Handed Duplexes with Z-Form-like Structure”, Journal of the American Chemical Society, 1984, vol. 106, pp. 3675-3675.
Sugiyama et al., “Synthesis, structure and thermodynamic properties of 8-methylguanine-containing oligonucleotides: Z-DNA under physiological salt conditions”, Nucleic Acids Research, 1996, vol. 24, No. 7, pp. 1272-1278.
Xu et al., “8-Methylguanosine: A Powerful Z-DNA Stabilizer”,J. Am. Chem. Soc., 125:13519-13524 (2003).
Oyoshi et al., “Efficient C2 α-Hydroxylation of Deoxyribos e in Protein-induced Z-Form DNA”,J. Am. Chem. Soc., 125:1526-1531 (2003).
Kawai et al., “Conformation-Dependent Photochemistry of 5-Halouracil-Containing DNA: Stereospecific 2′α-Hydroxylation of Deoxyribose in Z-form DNA”,J. Am. Chem. Soc., 121:1391-1392 (1999).

Affiliated with

Also associated with

Rating

Guanosine derivatives and use thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Guanosine derivatives and use thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Guanosine derivatives and use thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4065795