GSK3 polypeptides

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase

Reexamination Certificate

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C435S069100, C435S194000, C435S325000, C435S252300, C435S320100, C435S007100, C435S365000, C435S006120, C435S007600, C435S007800, C435S007920, C436S086000, C436S512000, C530S350000, C530S388100, C536S023200, C536S023500

Reexamination Certificate

active

10733816

ABSTRACT:
The invention provides truncated GSK3 polypeptides capable of crystallization, including GSK3α and GSK3β polypeptides, and use of these polypeptides to identify and optimize GSK3 inhibitors. Also provided are GSK3 polypeptides having at least one substituted amino acid that differs from wild-type GSK3, wherein the substituted amino acid is incapable of being phosphorylated. The invention finds use in providing methods of identifying and optimizing compounds useful for treating diseases mediated by GSK3 activity, including Alzheimer's disease, type 2 diabetes, and inflammation.

REFERENCES:
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