Growth medium containing a complex of prealbumin, a retinoid and

Details Growth medium containing a complex of prealbumin, a retinoid and Growth medium containing a complex of prealbumin, a retinoid and

1995-06-02

1996-05-21

Withyshyn, Michael G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352402, 435 701, 435 703, 435 704, 530399, 530837, 530838, C12N 500, C12P 2104

Patent

active

055189148

ABSTRACT:
This invention provides a complex consisting essentially of prealbumin, retinol-binding protein, and a retinoid which binds to retinol-binding protein, wherein the concentration of retinoid in the complex is from about 2.times.10.sup.-5 M to about 10.sup.-10 M and the concentration of prealbumin and retinol-binding protein is sufficient to maintain the retinoid concentration from about 2.times.10.sup.-5 M to about 10.sup.-10 M. This invention also provides a composition for enhancing the growth of human B cells in culture which comprises an amount of the complex of this invention effective to enhance the growth of human B cells and a nutrient medium. This invention further provides a method of enhancing growth of human B cells in culture comprising growing the cells in culture in a growth medium containing a concentration of the complex effective to enhance growth of the cells. This invention also provides a synthetic growth medium for culturing animal cells comprising the complex of this invention at a concentration effective to enhance cell growth. This invention also provides a method of preventing the growth of human tumor cells containing growth factor which comprises removing the growth factor from the human tumor cells.

REFERENCES:
Ambrus et al., "Identification of a cDNA for a Human High-Molecular-Weight B-cell Growth Factor", Proc. Natl. Acad. Sci. USA 90:6330-6334, Jul. 1993.
Buck et al., "Differences in the Action and Metabolism Between Retinol and Retinoic Acid in B Lymphocytes," J. Cell Biology 115(3):851-859.
Goodman, D. S., "Plasma Retinol-Binding Protein," in The Reintoids, vol. 2, Academic Press, pp. 41-88.
Buck et al., "Purification and Biochemical Characterization of a Human Autocrine Growth Factor Produced by Epstein-Barr Virus Transformed B Cells", Journal of Immunology, v. 138, 2923-2928, May 1, 1987.
Ambrus et al. (A), "Human B Rymphoma cell line producing B cell growth factor", Journal of Clinical Investigation, v. 75, 732-739, 1985.
Ambrus et al. (B), "Purification to homogeneieth of a high molecular weight human B cell growth factors", J. Exp. Medicine, v. 162, 1319-1335, 1985.
Campbell, Laboratory Techniques: Monoclonal Antibody Technology, pp. 104.105, 1984.

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