Growth media and assay for Yersinia enterocolitica

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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4352403, 43524031, 4352521, C12N 100, C12N 120, C12N 500, C12N 502

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active

054478499

ABSTRACT:
A growth medium for enhancing the selective growth of most serotypes of Yersinia enterocolitica obtained from environmental and clinical samples. The medium comprises an effective amount of a composition of a suitable nutrient source for Y. enterocolitica and an effective antimicrobial amount of 5-chloro-2-(2,4-dichlorophenoxy) phenol. The medium components may be provided as a dry powder for use in an aqueous formulation. An improved assay and isolation method is provided.

REFERENCES:
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Difco Manual, 10th Ed. Difco Laboratories, Detroit, Mich. pp. 430-431, 1055-1056, 1115, 1141-1143 (1984).
Greenwood, et al. Epid. Inf. v 101 pp. 53-58 (1988).
Difco Manual 10th Ed. Difco Laboratories Detroit, Mich. p. 1020 (1984).
PEI Food Tech Center Aug. 3, 1993 (see 3 pages).
Wauters et al: "New enrichment method for isolation of pathogenic Yersinia enterocolitica serogroup 0:3 from Pork", Applied and Environmental Microbiology 34, (4), 851-854 (1988).
Schiemann: "Development of a two-step enrichment procedure for recovery of Yersinia enterocolitica from food", Applied and Environmental Microbiology, 43, (1), 14-27 (1982).
Kounev: "Procedures for recovery of stressed and injured cells of Yersinia enterocolitica from meat and meat products", Journal of Food Protection, 52, (5), 360-362 (1989).
Landgraf et al: "An improved enrichment procedure for the isolation of Yersinia enterocolitica and related species from milk", Journal of Food Protection,56 (5) 447-450 (1993).
Kounev: "Effects of enrichment medium and incubation temperature on recovery of Yersinia enterocolitica from cooked sausage", Journal of Food Protection, 52 (11), 818-820 (1989).

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