Growing Ehrlichia species in a continuous cell line

Details Growing Ehrlichia species in a continuous cell line Growing Ehrlichia species in a continuous cell line

1990-05-03

1993-03-09

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

4352401, 43524021, 435260, C12N 500, C12N 104

Patent

active

051926792

ABSTRACT:
The present invention relates to a method of continually propagating Ehrlichia canis in a microphage-monocyte cell line referred to as DH82.

REFERENCES:
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Wellman, A Macrophage-Monocfyte Cell Line from a Dog with Malignant Histiocytosis; In Vitro Cellular & Developmental Biology, vol. 24, No. 3, Part I, pp. 223-229, Mar., 1988.
Rikihisa, Yasuko, Growth of Ehrlichia risticii in Human Colonic Epithelial Cells; Annals New York Academy of Sciences, 590:104-110 (1990).
Hemelt, et al.,. Serial Propagation of Ehrlichia canis in Primary Canine Peripheral Blood Monocyte Cultures; Cornell Vet., 70:38-42 (1980).
Nyindo, et al., Tropical Canine Pancytopenia: In Vitro Cultivation of the Causative Agent-Ehrlichia canis; American Journal of Veterinary Research, vol. 32, pp. 1651-1658, 1971.
Dawson, et al., Serologic Diagnosis of Human Ehrlichiosis Using Two Ehrlichia canis Isolates; Journal of Infectious Diseases, vol. 163, pp. 564-567, 1991.
Holland, et al., Isolation, Experimental Transmission, and Characterization of Causative Agent of Potomac Horse Fever; Science, vol. 227, pp. 522-524, Feb., 1985.
Ristic, et al., Diagnosis of Equine Monocytic Ehrlichiosis (Potomac Horse Fever) by Indirect Immunofluorescence; JAVMA, vol. 189, No. 1, pp. 39-467, Jul., 1986.
Ristic, Miodrag, Pertinent Characteristics of Leukocytic Rickettsiae of Humans and Animals In Microbiology (ed.:Leive) American Society for Microbiology, pp. 182-187, 1986.
Holland, et al., Adaptation of Ehrlichia sennetsu to Canine Blood Monocytes: Preliminary Structural and Serological Studies with Cell Culture-Derived Ehliichia sennetsu; Infection and Immunity, pp. 366-371, May, 1985.
Cole, et al., Continuous Propagation of Ehrlichia sennetsu in Murine Macrophage Cell Cultures; Am. J. Trop. Med. Hyg., 34(4), pp. 774-780, 1985.
Stephenson, et al., Somatic Cell Hybrids of Canine Peritoneal Macrophages and SV40-Transformed Human Cells: Derivation, Characterization, and Infection with Ehrlichia canis; Am. J. Vet. Res., vol. 41, No. 2, pp. 234-240, Feb., 1980.
Smith, et al., Isolation in Illinois of a Foreign Strain of Ehrlichia canis, The Causative Agent of Canine Ehrlichiosis (Tropical Canine Pancytopenia); JAVMA, vol. 166, No. 2, pp. 172-1745, 1975.
Brown, et al., In Vitro Cultivation of Neorickettsia helminthoeca, The Causative Agent of Salmon Poisoning Disease; Am. J. Vet. Res., vol. 33, No. 8, pp. 1695-1700, 1972.

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