Gridlock nucleic acid molecules, polypeptides, and...

Chemistry: molecular biology and microbiology – Vector – per se

Reexamination Certificate

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C530S350000, C536S023100

Reexamination Certificate

active

06991936

ABSTRACT:
The invention provides a novel gene, gridlock, and its encoded protein. gridlock plays a role in vascular development and modeling, and a mutation in gridlock has been associated with an aortic arch disease, coarctation. Thus, gridlock nucleic acid molecules and polypeptides can be used in methods of diagnosing, treating, and preventing gridlock-related diseases and conditions, such as aortic arch diseases.

REFERENCES:
patent: WO 01/72777 (2001-10-01), None
patent: WO 02/31111 (2002-04-01), None
Weinstein et al. Gridlock, a localized heritable vascular patterning defect in zebrafish Nature medicine 11:1143-1147 1995.
Chin et al., “Cardiovascular Basic Helix Loop Helix Factor 1, a Novel Transcriptional Repressor Expressed Preferentially in the Developing and Adult Cardiovascular System,” Journal of Biological Chemistry 275:6381-6387 (2000).
Fisher et al., “The WRPW Motif of the Hairy-Related Basic Helix-Loop-Helix Repressor Proteins Acts as a 4-Amino-Acid Transcription Repression and Protein-Protein Interaction Domain,” Molecular and Cellular Biology 16:2670-2677 (1996).
Kokubo et al., “Identification and Expression of a Novel Family of bHLH cDNAs Related to Drosophila Hairy and Enhancer of Split,” Biochemical and Biophysical Communications 260:459-465 (1999).
Leimeister et al., “Hey Genes: A Novel Subfamily of Hairy and Enhancer of Split Related Genes Specifically Expressed During Mouse Embryogenesis,” Mechanisms of Development 85:173-177 (1999).
Stainier et al., “Mutations Affecting the Formation and Function of the Cardiovascular System in the Zebrafish Embryo,” Development 123:285-292 (1995).
Weinstein et al., “Gridlock, a Localized Heritable Vascular Patterning Defect in Zebrafish,” Nature Medicine 11:1143-1147 (1995).
Zhong et al., “Gridlock, an HLH Gene Required for Assembly of the Aorta in Zebrafish,” Science 287:1820-1824 (2000).
Gessler, “Homo sapiens mRNA for Basic-Helix-Loop-Helix Protein, bHLH (Hey 2 Gene),” Sep. 30, 1999, Database EMBL [Online] HSA249545, Accession No. AJ249545, Abstract.
Lovell, Human DNA Sequence from Clone RP1-293L8, Jun. 3, 1999, Database EMBL [Online] HSDJ293L8, Accession No. AL078594.14, Abstract.
Nakagawa, “HRT1, HRT2, and HRT3: A New Subclass of bHLH Transcription Factors Marking Specific Cardiac, Somitic, and Pharyngeal Arch Segments,” Developmental Biology 216:72-84, 1999.

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