Green and orange fluorescent labels and their uses

Details Green and orange fluorescent labels and their uses Green and orange fluorescent labels and their uses

2007-12-29

2010-02-23

Powers, Fiona T (Department: 1626)

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

C558S017000, C562S052000, C562S055000, C436S172000, C436S800000

Reexamination Certificate

active

07667048

ABSTRACT:
The present invention provides novel fluorescent compounds and covalent attachment chemistries which facilitate the use of these compounds as labels for ultrasensitive and quantitative fluorescent detection of low levels of biomolecules. In a preferred embodiment, the fluorescent labels of this invention are novel derivatives of the hydroxy-pyrene trisulphonic and disulphonic acids which may be used in any assay in which radioisotopes, colored dyes or other fluorescent molecules are currently used. Thus, for example, any assay using labeled antibodies, proteins, oligonucleotides or lipids, including fluorescent cell sorting, fluorescence microscopy (including dark-field microscopy), fluorescence polarization assays, ligand, receptor binding assays, receptor activation assays and diagnostic assays can benefit from use of the compounds disclosed herein.

REFERENCES:
patent: 4844841 (1989-07-01), Koller et al.
patent: 5132432 (1992-07-01), Haugland et al.
patent: 5514710 (1996-05-01), Haugland et al.
patent: 5569366 (1996-10-01), Chen et al.
patent: 6653141 (2003-11-01), Singaram et al.
patent: 6949632 (2005-09-01), Conrad et al.
patent: 7317111 (2008-01-01), Bhatt et al.
patent: 2005/0123935 (2005-06-01), Haugland et al.
patent: 1 441 021 (1976-06-01), None
patent: WO 90/05916 (1990-05-01), None
Baustert, J.H. et al. (1988). “Fluorometric Continuous Assay of α-Chymotrypsin Using new Protease Substrates Possessing Long Wavelength Excitation and Emission Maxima.”Analytical Biochemistry. vol. 171, pp. 393-397.
Chen, F. et al. (1995) “Analysis of mono- and oligosaccharide isomers derivatized with 9-aminopyrene—1,4,6- trisulfonate by capillary electrophoresis with laser-induced Fluorescence.”Analytical Biochemistry. vol. 230, pp. 273-280.
Chiesa, C. et al., (1994) “Capillary zone electrophoresis of oligosaccharides derivatized with various aminonaphthalene sulfonic acids.”Electrophoresis. vol. 15, pp. 1132-1140.
Csortos, C. et al. (1990) “Interaction of the Catalytic Subunits of Protein Phosphatase-1 and 2-A with Inhibitor-1 and 2: A fluorescent Study with Sulfhydryl specific Pyrene Maleimide.”BBRC. vol. 169, No. 2, pp. 559-564.
Evangelista, R.A. et al. (1996) “Acid-catalyzed reductive amination of aldoses with 8-amino-pyrene-1,3,6-trisulfonate.”Electrophoresis. vol. 17, pp. 347-351.
Guttman, A. et al., (1995) “Capillary gel electrophoresis separation of high-mannose type oligosaccharides derivatized by 1-aminopyrene-1,6,8-trisulfonic acid.”Electrophoresis. vol. 16, pp. 1906-1911.
Jackson, P. (1990) “The use of polyacrylamide-gel electrophoresis for the high resolution separation of reducing saccharides labeled with the fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid.”Biochem. J. vol. 270, pp. 705-713.
Karim, A.S. et al. (1995) “Maleimide-mediated protein conjugates of nucleoside triphosphate gamma-S and an internucleotide phosphorothiate diester.”Nucleic Acids Research. vol. 23, No. 11, pp. 2037-2040.
Koller E. et al. “Longwave Absorbing and Fluorescing Fluorophore for Use in Hydrolase Analysis,”Applied Fluorescence Technology, Oct. 1989, pp. 15-16, vol. 1, No. 5.
Weltman, J.K. et al. (1973) “N-(3-pyrene) maleimide: a Long Lifetime Fluorescent Sulfhydryl Reagent.”J. Biol. Chem. vol. 248, No. 9, pp. 3173-3177.

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