GreA

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 701, 435 717, 435325, 4353201, 4352523, 43525411, 536 231, 536 237, C12N 120, C12N 1500, C12N 1511

Patent

active

058663681

ABSTRACT:
The invention provides greA polypeptides and DNA (RNA) encoding greA polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing greA polypeptides to screen for antibacterial compounds.

REFERENCES:
Borukhov, et al., GenBank Submission, Accession No. U81522, "Transcript cleavage factors from E. Coli ", 2 Jan. 1997.
Fraser et al., GenBank Submission, Accession No. U397108 L42023, "The minimal gene complement of Mycoplasma genitalium.", 21 Apr. 196.
Fleischmann, et al., GenBank Submission, Accession No. U32812 L42023, "Whole-genome random sequencing and assembly of Haemophilus influenza Rd."27 Sep. 1996.
Fleischmann, et al., GenBank Submission, Accession No. U32738 L42023, "Whole-genome random sequencing and assembly of Haemophilus influenza Rd.", 27 Sep. 1996.
Wang, et al., GenBank Submission, Accession No. U01376, M93424, "Identification and physical analysis of new genes in the argG region (69 min) of Escherichia coli chromosone.", 25 Apr. 1996.
Himmelreich, et al., GenBank Submission, Accession No. AE000043, U00089, "Complete sequence analysis of the bacterium Mycoplasma pneumoniae".
Marks, et al., GenBank Submission, Accession No. Z12122, "Direct Submission", 16 Jul. 1992.
Altmann, et al., "RNA cleavage and chain elongation by Escherichia coli DNA-dependent RNA polymerase in binary enzyme-RNA complex." Proc. Natl. Acad. Sci. U.S.A., Vol. 91, pp. 3784-3788, Apr. 1994.
Borukhov, et al., "GreA protein: A transcription elongation factor from Escherichia coli," Proc. Natl. Acad. Sci., U.S.A., vol. 89, pp. 8899-8902, Oct. (1992).
Feng, et al., "DreA-induced Transcript Cleavage in Transcription Complexes Containing Escherichia coli RNA polymerase Is Controlled by Multiple Factors, Including Nascent transcript Location and Structure." The Journal of Biological Chemistry, vol. 269, No. 35, Issue of Sep. 2, pp. 22282-22294, (1994).
Hsu, et al., "Escherichia coli transcript clevage factors GreA abd GreB stimulate promotor escape and gene expression in vivo and in vitro." Proc. Natl. Acad. Sci. , U.S.A., vol. 92, pp. 11588-11592,Dec., (1995).
Koulich, et al., "Domain Organization of Escherichia coli Transcript Cleavage Factors GreA and GreB." The Journal of Biological Chemistry, vol. 272, No. 11, Issue Mar. 14, pp. 7201-7210, (1997).
Lu, et al., "Identification of greA encooding a Transcriptional Elogation Fator as a member of the carA-orf-carB-greA Operon in Pseudomonas earuginosa PAO1."Journal of Bacteriology, vol. 179, No. pp. 3043-3046, May (1997).
Nudler, et al., "Coupling between Transcription termination and RNA Polymerase Inchworming."Cell, vol. 81, pp. 351-357, May 5, (1995).
Sparkowski, et al., "The nucleotide sequence of greA, a suppressor gene that restores growth of an Escherchia coli RNA polymerase mutant at high temperature."Nucleic Acids Research, vol. 18, No. 21, pp. 6443 (1990).
Severinov, et al., "Topology of the Products Binding Site in RNA Polymerase Revealed by Transcript Slippage at the Phage .lambda. P.sub.L Promotor." Journal of Biological Chemistry, vol. 269, No. 50, pp. 31710-31705, Isueu of Dec., (1994).
Stebbins, et al., "Crystal Structure of the GreA transcript cleavage factor from Escherichia coli." Nature, vol. 373, pp. 636-640, Feb. 16, (1995).
Wang, et al., "Nuclease Cleavage of the Upstream Half of the Nontemplste Strand DNA in an Escherichia coli Transcription Elongation complex Causes Upstream Translocation and transcriptional Arrest." The Journal of Biological Chemistry, vol. 272, No. No. 9, pp. 5989-5994, (1997).
Orlova, et al., "Intrinisic transcript cleavage activity RNA polymerase." Proc.Nstl. Acad. Sci., vol. 92, 4596, pp. 4596-4600, May, (1990).
Erie, et al., "Multiple RNA Polymerase Confirmations and GreA: Control of the Fidelity of Transcription." Science, vol. 262, Nov. 5, (1993).
Sparkowski, et al., "Location of a New Gene, greA, on the Escherichia coli, Chromosone." Journal of Bacteriology, vol. 173, No. 17, pp. 5256-5257, (1991).
Borukhov, et al., "Transcript Clevage factor from E. Coli."Cell vol. 72, pp. 459-466, Feb. 12, 1993.
Komissarova, et al., "Transcriptional arrest: Escherchia coli RNA polymerase translocated backward leaving the 3' end of the RNA intact and extruded." Proc. Natl. Acad. Sci. , vol. 94, pp. 1755-1760, Mar. (1997).
Lee, et al., "GreA-induced Transcript Clevage Is Accompanied by Reverse Translocation to a Different Transcription Complex Conformation."The Journal of Biological Chemistry, vol. 269, No. 35, pp. 22295-22303 (1994).

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