Gram negative bacteria screening method with horseshoe crab ameb

Details Gram negative bacteria screening method with horseshoe crab ameb Gram negative bacteria screening method with horseshoe crab ameb

1985-12-03

1988-01-05

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 436502, G01N 33579

Patent

active

047176580

ABSTRACT:
A screening method for the determination of 10.sup.4 gram negative bacteria per milliliter of an undiluted urine sample and a unitary screening test device for the determination of at least 10.sup.5 gram negative bacteria per milliliter of an undiluted urine sample. The method and test device make use of Limulus amebocyte lysate (LAL) and a synthetic substrate containing a chromogenic or fluorogenic leaving group capable of being cleaved by activated lysate. This screening method and unitary screening device provide a quick, convenient, inexpensive indication of the possible presence of a urinary tract infection caused by gram negative bacteria.

REFERENCES:
patent: 4188264 (1980-02-01), Iwanaga
patent: 4276050 (1981-06-01), Firca
patent: 4301245 (1981-11-01), Lindsay
Chemical Abstracts, I, 102: 1464r (1985).
Chemical Abstracts, II, 102: 180282z (1985).

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